Vol 5, No 3 (2018) > Articles >

Pengaruh Variasi Konsentrasi Hidroxy Propyl Methyl Cellulose (HPMC) terhadap Stabilitas Fisik Gel Ekstrak Tembakau (Nicotiana tabaccum L.) dan Aktivitasnya terhadap Streptococcus mutans

Kori Yati , Mahdi Jufri , Misri Gozan , Mardiastuti Wahid , Lusi Dwita

 

Abstract:

Tobacco extract had been proven to have antimicrobial activity against Streptococcus mutans. To maximize the use of tobacco extract on topical use as an antibacterial, it could be formulated into a pharmaceutical preparation. This study aimed to formulate tobacco extract in gel preparation by using Hidroxy Propyl Methyl Cellulose (HPMC) as a gelling agent and to test its activity on S. mutans. The tobacco extract gel was prepared in 3 formulas with variations of HPMC concentration of 1.5% (F1), 2% (F2) and 2.5% (F3). The research began with tobacco extraction, then continued with characteristics evaluation. The extract was formulated in gel form and evaluated for 12 weeks of physical stability. Antibacterial activity was tested using the diffusion method. The evaluation results of tobacco extract gel showed that all formulas were stable during 12 weeks storage. Antimicrobial activity against S.mutans showed inhibitory diameter of F1, F2 and F3, were 9,07 mm, 19,53 mm, and 11,57 mm respectively. The test was continued by determining the relative potential of F2 compared to erythromycin. The test results showed 1.2 x 10-2 relative potential compare to erythromycin. Based on the results of this study, it can be concluded that HPMC concentration difference did not give significant difference to the physical stability of tobacco gel, with the best antibacterial activity on S. mutans obtained from F2.


Keywords: tembakau extract; gel; HPMC; Streptococcus mutans

Published at: Vol 5, No 3 (2018) pages: 133-141

DOI: 10.7454/psr.v5i3.4146


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References:

Allen LV. (1998) The art and technologi of pharmaceutical compounding, American Pharmaceutical Association, Whashington DC, 322-323.

Departemen Kesehatan RI. (1995). Farmakope Indonesia edisi IV. Jakarta. Hlm. 413,551, 618, 687, 712-713, 756.

Dwirara J. (2016) Aktivitas ekstrak etil asetat kulit buah manggis sebagai antibiofilm S. mutans. Skripsi. Jakarta Timur: Universitas Muhammadiyah Prof. DR HAMKA (UHAMKA).

Fatimah IA, Kusumawardani B, Meilawaty Z, & D A W S. (2016). Pengaruh ekstrak flavonoid rendah nikotin limbah daun tembakau kasturi ( Nicotiana tabaccum L .) terhadap pertumbuhan mikroba rongga mulut. Artikel Ilmiah Hasil Penelitian Mahasiswa.

Fauzantoro A, Amatullah A, Muharram Y, Gozan M. (2016). Production of Nicotiana tabacum L. extract: from laboratory-to pilot-scale and its antifungal activity against Aspergillus niger. Proceedings and Book Abstracts Biotechnology International Congress (BIC). Bangkok, 20-23 September 2016.

Fauzantoro A, Muharam Y, & Gozan M. (2017). Improvement of nicotine yield by ethanolic heat reflux extraction of Nicotiana tabacum var . Virginia Origin of Ponorogo. International Journal of Applied Engineering Research, 12(23), 13891–13897.

Gandhi P T, Narayanappa T, & Ramesh G. (2016). Bioorganic & Medicinal Chemistry Novel nicotine analogues with potential anti-mycobacterial activity. Bioorganic & Medicinal Chemistry, 24(8), 1637–1647. https://doi.org/10.1016/j.bmc.2016.02.035.

Garg AD, Aggarwal S, Garg and AK Sigla. (2002). Spreading of semisolid formulation: An update. Pharmaceutical Tecnology. September: 84-102.

Jufri M, Irmayanti E, & Gozan M. (2016). Formulation of tobacco based mosquito repellent to avoid dengue fever. International Journal of Pharm Tech Research, 9(7), 140–145.

Lachman L, Liebermen HA, Kaning JL. (1994). Teori dan Praktek FarmasiIndustri. Edisi II. (Siti Suyatmi). Jakarta: UI Press. Hlm. 1029-1081.

Listiyati A K, Nurkalis U, & Hestiningsih R. (2012). Ekstraksi nikotin dari daun tembakau (Nicotina tabacum) dan pemanfaatannya sebagai insektisida nabati pembunuh aedes Sp. Jurnal Ilmiah Mahasiswa, 2(2), 67–70.

Mansjoer A. (2000). Kapita Selekta Kedokteran, Edisi III Jilid II. Jakarta: Media Aesculapius, FKUI.

Nevi S. (2006). Formulasi sabun transparan minyak nilam sebagai obat jerawat. Jakarta: UHAMKA.

Prakash B, Veeregowda BM, Krishnappa G. (2003). Biofilms: A survival strategy of bacteri. Current Science. 85(9): 1299-1307.

Puspita P E. (2011). Aktivitas antibakteri ekstrak tembakau temanggung varietas genjah kemloko. Institut Pertanian Bogor.

Putri RH, Barid I, Kusumawardani B, Gigi F K, Jember U, Ilmu, B., … Jember, U. (2014). daya hambat ekstrak daun tembakau terhadap pertumbuhan mikroba rongga mulut. Stomatognatic, 11(2), 27–31.

Rowe, Paul J dan Sian C. (2006). Handbook of Pharmaceutical Excipients FifthEdition. American Pharmacists Assosiation. Washington. Hlm. 466-469,581-584, 629-632, 624-625.

Sayuti NA. (2015). Formulasi dan Uji Stabilitas Fisik Sediaan Gel Ekstrak Daun Ketepeng Cina (Cassia alata L.). Jurnal Kefarmasian Indonesia Volume 5 No 2. p. 74-82.

Seneviratne CJ, Zhang CF, Samaranayake LP. (2011). Dental Plague Biofilm in jOral Health and Disease. The Chinese Journal of Dental Research. 14(2): 87-94.

Selviana M. I. Tigauw, C. L. S. dan J. M. (2015). Efektivitas Ekstrak Bawang Putih dan tembakau Terhadap Kutu Daun (Myzus persicae Sulz.) pada Tanaman Cabai(Capsicum sp.). Eugenia, 21(3), 135–141.

Susilowati, E. Y. (2006). Identifikasi Nikotin dari Tembakau (Nicotiana tabacum) Kering dan Uji Efektivitas Ekstrak Daun Tembakau sebagai Insektisida Penggerek Batang Padi (Scirpophaga innonata).

Voigt R. (1994). Buku Pelajaran Teknologi Farmasi. Terjemahan: Soendani Noerono. Universitas Gajah Mada Press. Yogyakarta. Hlm. 116-118, 607-608, 578-583.

Yosipovitch G, Greaves MW, Schmelz M. (2003). The Importance of Skin pH. Department of Dermatology: Wake Forest University. Vol 361, No. 935.