Vol 5, No 3 (2018) > Articles >

Aktivitas Antiinflamasi Ekstrak Etanol Daun Karas (Aquilaria malaccensis Lamk.)

Pratiwi Apridamayanti , Ferlino Sanera , Robiyanto Robiyanto

 

Abstract:

Karas (Aquilaria malaccensis Lamk.) leaves contain secondary metabolite such as alkaloid, flavonoid, phenol, antraquinone, and triterpenoid. Flavonoid compound has anti inflammatory activity. This research was conducted to investigate the effective anti inflammatory dose from the reduction of rat paw edema using plethismometer. Karas leaves was macerated with 96% ethanol and then evaporated until crude extract was obtained. This research was carried out using 25 male rats that was divided into 5 treatment groups, negative control (CMC-Na 1%), positive control (Natrium diclofenac 4.5 mg/kgBW), dosage I (45 mg/kgBW), dosage II (90 mg/kgBW), and dosage III (180 mg/kgBW). The extract was administrated orally half an hour before the induction of 0.1 ml carragenan 2% solution. The anti inflammatory activity was observed from the volume of edema, AUC, and the percentage of antiinflammatory activity. The data was analyzed by ANOVA using SPSS. The result shows that there was a significant difference between negative control with the treatment groups (dosage I, II, and III). There was no significant difference between positive control with dosage II and III, however there was a significant difference to dosage I. The percentage of antiinflammatory activity of positive control, dosage I, dosage II, and dosage III was 39.3%, 22.9%, 29.6%, and 37.9% respectively. The conclusion of this research was that the effective dose of ethanolic extract form karas leaves was 180 mg/kgBB.


Keywords: ethanol ekstract karas leaves; Aquilaria malacencis Lamk.; antiinflamatory activity

Published at: Vol 5, No 3 (2018) pages: 152-158

DOI: 10.7454/psr.v5i3.4094


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References:

Alam J, Mujahid M, Badruddeen, Rahman MA, Akhtar J, Khalid M, et al. (2015). An insight of pharmacognostic study and phytopharmacology of Aquilaria agallocha. Journal of Applied Pharmaceutical Science, 5(08), 173-181

Baratawidjaja KG & Rengganis I. (2013). Imunologi dasar. Edisi 10. Fakultas Kedokteran Universitas Indonesia. Jakarta: 219, 275-276

Departemen Kesehatan Republik Indonesia. (2008). Farmakope herbal Indonesia edisi 1. Departemen Kesehatan Republik Indonesia, Jakarta

Dugowson EC, and Gnanashanmugam P. (2006). Nonsteroidal anti-inflammatory drugs. Physical Medicine and Rehabilation, 1, 347-357

Ganiswarna SG. (1995). Farmakologi dan terapi. Bagian Farmakologi Fakultas Kedokteran Universitas Indonesia, Jakarta

Guttman E, Nograles KE, Krueger JG. (2011). Contrasting pathogenesis of atopic dermatitis and psoriasis part II: Immune cell subsets and therapeutic conceps. Journal Allergy Clin Immuno, 127, 1420-32

Hamid KHK, Mustafar NS, Yusof PM, Shawal SSM, Musa M, and Rodhi MMM. (2015). Determinan of compounds inside inoculoated aqularia malaccensis leaves by soxhlet extraction. Advanced material research, 1113

Harborne JB. (2006). Metode fitokimia penentuan cara modern menganalisis tumbuhan, Institut Teknologi Bandung. Bandung

Huda AWN, Munira MAS, Fitrya SD, and Salmah M. (2009). Antioxidant activity of aquilaria malaccensis (thymalaeceae) leaves. Pharmacognosy research, 1(5), 270-273

Juheini FW, Mariana Y, dan Rusmawan I. (1990). Efek antiinflamasi jahe (Zingiber officinale rose.) terhadap radang buatan pada tikus putih. Majalah Farmakologi dan Terapi Indonesia, 7(1), 9-13

Kim HP, Mani I, Iversen L, and Ziboh VA. (1998). Effects of Naturally-Occurring Flavonoids and Biflavonids on Epidermal Cyclooxygenase and Lipoxygenase from Guinea-Pigs. Prostaglandins, Leukotriences and Essential Fatty Acids, 58(1), 17-24

Kristanti AN, Aminah NS, Tanjung M, Kurniadi B. (2010). Buku ajar fitokimia. Airlangga University Press, Surabaya

Lemens RHMJ and Bungapraphatsura M. (2003). Plant resources of south east asia. Jilid 12. (edisi 3). Medical and Poisonous Plant 3. Backuys Publisher, Leiden

Mycek MJ. (2001). Farmakologi ulasan bergambar. Edisi 2. Widya Medika, Jakarta

Nijveldt R, Nood EV, Hoorn DEV, Boelens PG, Norren KV, and Leeuwen PAV. (2001). Flavonoids: a riview of probable mechanisms of action and potential applications. American Journal of Clinical and Nutrition, 74. 418-425

Sujono TA, Patimah R, Yuliani R. (2012). Efek antiinflamasi infusa rimpang temu putih (Curcuma zeodoria) pada tikus yang diinduksi karagenan. J Biomedika, 4(2), 10-17

Wang S, Yu Z, Wang C, Wu C, Gou P, and Wei J. (2018). Chemical constituen and pharmacological activity of agarwood and Aquilaria plants. Molecules, 23, 342

Yadav DK, Mudgal V, Agrawal J, Maurya AJ, Bawankule DU, Chanotiya CS, et al. (2013). Molecular docking and ADME studies of natural compouns of agarwood oil for topical anti-inflammatory activity. Curr.Comput, Aided Drug, 9, 36-370

Zhou M, Wang H, Suolangjiba, Kou J, Yu B. (2008). Antinociceptive and anti-inflammatory activities of Aquilaria sinensis (Lour.) Gilg. leaves extract. J Ethnopharmacol, 117, 345-350.