Vol 4, No 1 (2007) > Articles >

UJI AKTIVITAS PENGHAMBATAN ENZIM A-GLUKOSIDASE DAUN KALANCHOE PINNATA PERS.

Efizal Efizal , Atiek Soemiati , M. Hanafi , Indah Dwiyanti

 

Abstract: The study of inhibitory activity against the a-glucosidase enzyme of Kalanchoe pinnata Pers. leaves has been carried out. In vitro, the methanol extract, n-hexane fraction, ethyl acetate fraction and methanol-water (1:1) fraction inhibited a-glucosidase enzyme activities with IC50 value 72.8, 982.6, 194.6 and 293.8 ug/mL, respectively. In vivo, the study was conducted in normal ddY mice by using oral glucose tolerance test. The administration of methanol extract dose 0.5, 1.0 g/kg of body weight and ethyl acetate fraction dose 1.0 g/kg of body weight can suppress the blood glucose level at 30 min significantly (p<0.05) if compared with control after oral administration of sucrose (3 g/kg of body weight), and their activity were similar with acarbose 0.01 g/kg of body weight (p>0.05).

Keywords: Kalanchoe pinnata Pers.; a-glucosidase inhibitor; hypoglycaemic activity; ddY mice; inhibitor a-glukosidase; aktivitas hipoglikemik; mencit ddY

Published at: Vol 4, No 1 (2007) pages: 37-47

DOI: 10.7454/psr.v4i1.3812


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