Vol 4, No 1 (2017) > Articles >

Skrining dan Evaluasi Aktivitas Kitinase dan Produksi N-Asetilglukosamin dari Sembilan Isolat Bakteri Lokal

Baitha Palanggatan Maggadani, Siswa Setyahadi, Harmita Harmita

 

Abstract:

Kitinase adalah suatu poli 1,4-β (2-asetamido-2-deoksi-D-glukosaminida), merupakan enzim yang mendegradasi kitin pada ikatan β-1,4-asetamido-2-deoksi-D-glikosida menjadi produk turunannya yaitu kitin oligosakaridaatau monomer NAG. Enzim kitinase dihasilkan oleh mikroorganisme   kitinolitik yang sebagian   besar   terdapat   di lingkungan  tanah  dan  air. Penelitian kali ini bertujuan untuk skrining dan evaluasi kemampuan 9 bakteri isolat dalam menghasilkan kitinase. Isolat yang terbaik adalah isolat yang menghasilkan kitnase dengan aktivitas yang tinggi dan mampu menghidrolisis kitin menjadi monomer NAG. Dari 9 isolat bakteri tersebut bakteri BPPTCC-2 merupakan bakteri yang terpilih karena mampu menghasilkan kitinase dengan aktivitas tertinggi yaitu 0,1780 U/mL  . kitinase tersebut dapat menghidrolisis substrat kitin dan menghasilkan NAG sebagai produk tunggal dan dengan  rendemen NAG paling tinggi yaitu 11,5%.

Keywords: Chitin, Chitinase, Enzymatic hydrolysis, N-acetylglucosamine, NAG
Published at: Vol 4, No 1 (2017) pages: 13-24
DOI:

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