Vol 4, No 1 (2017) > Articles >

Skrining dan Evaluasi Aktivitas Kitinase dari Sembilan Isolat Bakteri Lokal

Baitha Palanggatan Maggadani , Siswa Setyahadi , Harmita Harmita

 

Abstract:

Chitinase is an enzyme that degrades chitin on ?-1,4-acetamido-2-deoxy-D-glycoside bound to produce Chitin oligosaccharide or its soluble monomer, N-acetylglucosamine (NAG). Chitinase are usually produced naturally by chitinolytic microorganism that are found in soil or water environment. Chitinase have been widely used for biocontrol agents of plant pests. The product from chitinase hydrolysis can be used as antiinflammatory agent and anti hyperpigmentation. This research was aimed to screen and evaluate the chitinolytic ability of 9 isolate. The best isolate was the one which produced high chitinolytic activity and hydrolyzed chitin into NAG. 9 isolate were examined and BPPTCC-2 was the best isolate which produced highest level of chitinolytic activity 0.1780 U/ml. The chitinase was able to hydrolyzed chitin resulting high yields of NAG 11.5% as the only product.


Keywords: kitin; kitinase; hidrolisis enzimatik; N-asetilglukosamin; chitin; chitinase; enzymatic hydrolysis; N-acetylglucosamine

Published at: Vol 4, No 1 (2017) pages: 13-24

DOI: 10.7454/psr.v4i1.3650


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References:

Abd-Aziz S, Teoh LS, Alitheen N, Shahab N, Kamaruddin K. (2008). Microbial degradation of chitin materials by Trichoderma virens UKM1. Journal of biological sciences. 1, 52-59.

Aiba Seiichi. (2005). Production of N-acetyl-D-glucosamine from chitin using crude enzyme preparation in large scale. Journal of Metal, Material and Minerals. 2005. Vol 15 (1), 23-25.

Chang KL, Lee J, Wen FR. (2000). HPLC Analysis of N-acetyl-chito-oligosaccharides During the Acid Hidrolysis of Chitin. Journal of Food and Drug Analysis. 8(2), 75-83.

Chen JK, Shen CR, Liu CL. (2010) N-acetylglucosamine: Production and applications. Journal of Marine Drugs. 8,2493-2516.

Dahiya N, Tewari R, Hoondal GS. (2006). Biotechnological aspect of chitinolitic enzymes: a review. Appl Microbiol Biotechnol. 71,773-782.

Haliza W, Suhartono MT. (2012). Karakteristik kitinase dari mikroba. Buletin Teknologi Pascapanen Pertanian. Vol 8 (1).

Hamid R, Khan MA, Ahmad M, Ahmad MM, Musarrat J, Javed S. (2014). Chitinases: An Update. J Pharm Bioallied Sci. 5(1):21-29.

Herdyastuti N, Raharjo TJ, Mudasir, Matsjeh S. (2009). Kitinase dan mikroorganisme kitinolitik : isolasi, karakterisasi dan manfaatnya. Indo. J. Chem. 9 (1), 37 – 47.

Illanes A.(2008). Enzyme Biocatalysis. Principles and Application. Springer. Chille.

Jamialahmadi K, Bahravan J, Najafi FM, Yazdi TM, Shahverdi AR, Fahramarezi, AM . (2011). Enzymatic production of N-acetyl-D-Glucosamine from chitin using crude enzyme preparation of Aeromonas sp PTCC16491. Biotechnology. 10(3): 292-297.

Kuk JH, Jung WJ, Jo GH, Ahn JS, Kim KY, Park RD. (2004). Selective preparation of N-acetyl-D-glucosamine and N,N’-diacetylchitobiose from chitin using a crude enzym preparation from Aeromonas sp. Biotechnology letters. 27, 7-11.

Nagpure A, Choudhary B, Gupta RK. (2014). Chitinases: in agriculture and human healthcare. Critial Reviews in Biotechnology. Vol 3, Issue 3: 215-232.

Park SH, Lee JH, Lee HK. (2000). Purification and characterization of chitinase from a marine bacterium Vibrio sp. 98CJ11027. The Journal of Microbiology. 224-229.

Pratiwi RS, Susanto TE, Wardani YAK, Sutrisno A. (2014). Enzim kitinase dan aplikasi di bidang industri. Jurnal Pangan dan Agroindustri. Vol. 3 No 3 p. 878-887.

Sashiwa H, Fujishima S, Yamano, N, Kawasaki N, Nakayama A, Muraki E, et al. Production of N-Acetyl-D-glucosamine from α-Chitin by Crude Enzymes from Aeromonas hydrophila H2330. Carbohydr Res. 2002. 337, 761–763.

Sashiwa H, Fujishima S, Yamano N, Kawasaki N, Nakayama A, Muraki E. (2003). Enzymatic production of N-Acetyl-D-glucosamine from chitin. Degradation study of N-Acetylchitooligosaccharide and the effect of mixing of crude enzymes. Carbohydr. Polym. 51, 391–395.

Wang SL, Lin TY, Yen YH, Liao HF, Chen YJ. (2006). Bioconversion of shellfish chitin wastes for the production of Bacillus subtilis W-118 chitinase. Carbohydr Res. 341:2507-2515.

Wen CM, Tseng CS, Cheng CY, Li YK. (2002). Purification, characterization and cloning of a chitinase from Bacillus sp. NCTU2. Biotechnol. Appl. Biochem. Vol 35, 213-219.