Vol 5, No 1 (2018) > Articles >

Sensitivity of Escherichia coli Bacteria Towards Antibiotics in Patient with Diabetic Foot Ulcer

Rafika Sari , Pratiwi Apridamayanti , Indira Diah Puspita

 

Abstract: Chronic complication of diabetes mellitus is diabetic foot ulcers. Diabetic foot ulcer can be defined as an open wound in the feet which will become infected as the result of high blood sugar levels that develops and become place of bacteria. One of the bacteria in diabetic foot ulcer is Escherichia coli. Imprecise use of antibiotics in the treatment of diabetic foot ulcers can cause antibiotic resistance to bacteria. This study aims to determine antibiotic sensitivity of Escherichia coli  bacteria in diabetic foot ulcer Wagner grade III and IV. Samples of diabetic foot ulcer swab's with Wagner grade III and IV were taken as Consecutive sampling. Identification of Escherichia coli bacteria is done by using a biochemical test and Gram stain test. Antibiotic sensitivity test is conducted  by using Kirby Bauer's disc of diffusion method. Escherichia coli bacteria that were tested are sensitive to  amikacine, gentamicine, ciprofloxacin, levofloxacine, ceftriaxone, cefotaxime, imipenem and meropenem antibiotics but showed resistant to cefadroxil.

Keywords: diabetic foot ulcers; Escherichia coli; antibiotic sensitivity

Published at: Vol 5, No 1 (2018) pages: 19-24

DOI: 10.7454/psr.v5i1.3649


Access Counter: 194 views

Full PDF Download

References:

Anggriawan F, Endriani R, Sembiring LP. (2014). Identification of Gram Negative Bacteria Producing Extended Spectrum β Lactamase (ESBL) of Diabetic Ulcers Grade I and II Wagner in Ward Disease Arifin Achmad Hospital in Riau province. Riau University.

Aulia FN. (2008). Germs aerob patterns and sensitivity in diabetic gangrene. Thesis.

Cahyono JB. (2007). management of diabetic foot ulcers. Jakarta : Dexa.

Clinical and Laboratory Standards Institute. (2014). Performance standards for antimicrobial susceptibility testing; Twenty-fourth informational supplement. USA : Clinical and Laboratory Standards Institute.

Decroli E, Karimi J, Manaf A, Syahbuddin S. (2008). Profile diabetic ulcers in patients hospitalization in the department of internal medicine Dr. M. Djamil Hospital Padang. Indonesia Medicine Magazine, 58 (1), 5-7.

Frykberg RG. (2002). Risk factor, pathogenesis and management of diabetic foot ulcers. Iowa : Des Moines University.

Hasnawati, Sugito, Purwanto H, Brahim R. (2009). Profile indonesia health. Jakarta : Deparment of Health Republic Indonesia.

Indian Council of Medical Research (ICMR). (2009). Detection of antimicrobial resistance in common gram negative and gram positive bacteria encountered in infectious deseases-An update, ICMR Bulletin.

Kohanski MA, Dwyer DJ, Collins JJ. (2010). How antibiotics kill bacteria: from targerts to networks. Microbiology, 8, 423-435.

Lambert T. (2012). Antibiotics that affect the ribosome. Rev. sci. tech. Off. Int. Epiz, 31 (1), 57-64.

Paterson DL & Yu VL. (2005). Extended-spectrum β -lactamases: A clinical update. Clin Microbiol Rev , 18 (4), 657-686.

Radji M. (2012). Practical guidance pharmaceutical microbiology. Jakarta: University of Indonesia.

Rice LB & Bonomo RA. (1996). Genetic and biochemical mechanisms of bacterial resistance to antimicrobial agents in: Lorian, V (ed). Antibiotics in Laboratory Medicine. 4th ed. Maryland: Williams & Wilkins Co.

Rice LB. (1999). Successful interventions for gram-negative resistance to extended-spectrum β -lactam antibiotics. Pharmacotherapy, 19, 120-128.

Sari R & Apridamayanti P. (2015). Identification of ESBL producing bacteria in patients with diabetic foot ulcers grade III and IV wagner. Research Report DIPA Tanjungpura University.

Setiabudy R & Gunawan GS. (2007). Pharmacology and therapeutics ( 5th ed). Jakarta: Department of Pharmacology and Therapeutics Faculty of Medicine, University of Indonesia.

Soetjahjo A & Poerwadi T. (1991). Diabetic neuropathy: Classification, pathogenesis and therapy. In: Tjoksoprawiro A, Tandra H, eds. Surabaya: National Symposium on Development of Advanced Endocrinology Metabolism.

Wahab NH, Samsudin IN, Nordin SA, Ahmad Z, Noor LA, Devnani AS. (2015). Clinical presentation and microorganisms sensitivity profile for diabetic foot ulcers. Med J Malaysia, 70 (3), 182-187.

Widiyastutik VS, Wurlina, Budiarto. (2013). Sensitivity of Escherichia coli from goat milk peranakan etawa against antibiotics. Airlangga University ; 6(2).

Zubair M, Malik A, Ahmad J. (2010). Clinico-bacteriology and risk factors for the diabetic foot infection with multidrug-resistent microorganisms in North India. Biology and Medicine, 2 (4), 22-34.