Vol 3, No 3 (2016) > Articles >

Uji Aktivitas Anti-Inflamasi Minyak Atsiri Daun Kemangi (Ocimum americanum L.) pada Tikus Putih Jantan yang Diinduksi Karagenan

Fadlina Chany Saputri, M.Si, Apt , Rita Zahara

 

Abstract: Kemangi (Ocimum americanum L.) is an aromatic plant that contains citral and known as anti-inflammatory agent. The aim of this study was to determine the anti-inflammatory activity of the essential oil kemangi leaves (Ocimum americanum L.) on carrageenan-induced rat. The male rats were divided into six groups, each consisting of four rats. Group I as negative control was given 0.5% CMC solution, group II as positive control was given diclofenac sodium, group III was given sitral, groups IV, V and VI were given 40 mg/200 g BW, 80 mg/200 g BW, and 160 mg/200 g BW essential oil of Ocimum americanum L., emulsified in 0.5% CMC solution. After 30 minutes of test substance administration, left paw of rats injected by 0.2 mL of carrageenan to induce edema. Edema volume was measured using pletismometer every hour for six  hours. The result showed that at dose 160 mg/200 g BW gives the best effect in inhibited the inflamation response 44,83%. There was significant difference (p<0.05) at dose 160 mg/200 g BB to negative control. From this study can be concluded that  essential oil kemangi leaves has anti-inflammatory activity.

Keywords: Keywords: anti-inflammatory, carragenan, kemangi, Ocimum americanum L., citral

Published at: Vol 3, No 3 (2016) pages: 107-119

DOI: 10.7454/psr.v3i3.3619


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References:

Astuti, E.P. (2012). Pemisahan Sitral dari Minyak Atsiri Serai Dapur (Cymbopogon citratus) Sebagai Pelangsing Aromaterapi. IPB: Departeman Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam.

Chaimovitsh, D., et al. (2011). The Relative Effect of Citral on Mitotic Microtubules in Wheat Roots and BY2 Cells. Pubmed 14 (2), 354-644.

Corwin, E.J. (2008). Buku Saku Patofisiologi. Jakarta: Penerbit Buku Kedokteran EGC, 587.

Fadlianti, Delly. (2010). Kerakterisasi Simplisia Isolasi dan Analisis Komponen Minyak Atsiri dari Daun Kemangi Segar dan Kering (Ocimumi folium) secara GC-MS. Universitas Sumatera Utara :Fakultas Farmasi.

Fitriani, N.M. (2011). Uji Daya Antiinflamasi Ekstrak Etanol Daun Kemangi (Ocimum sanctum L.) pada Tikus Putih Jantan Galur Wistar. STIKES NWU: Farmasi.

Gard, Paul. (2001). Human Pharmacology (Chapter IX.). Taylor & Francis., London, New York, 135.

Goodman, L.S., and Gilman Alfred. (2011). The Pharmacological Basis of Therapeutics 12 th ed. Mc Graw-Hill Publication, 512-520.

Gunawan, D., dan Mulyani, S. (2004). Ilmu Obat Alam, Farmakognosi, Jilid I. Jakarta: Penerbit Penebar Swadaya, 107.

Hariana, Arief. (2005). Tumbuhan Obat dan Khasiatnya, seri 2. Jakarta: Penebar Swadaya, 26.

Irawan, I. (2008) Tanaman Lalap Berkhasiat Obat, cetakan IV Jakarta: Penerbit Penebar Swadaya, 42-45.

Jorge, Parada, C.A., Ferreira, S.H., and Tambeli, C.H. (2006). Interferential Therapy Produces Antinociception During Application in Various Models of Inflammatory Pain. Physical Therapy, 86(6), 800-8.

Katzung, B.G., Masters, S.B., and Trevor, A.J. (2009). Basic & Clinical Pharmacology, 11th Ed. New York: McGraw-Hill.

Kelompok Kerja Ilmiah. (1983). Penapisan Farmakologi, Pengujian Fitokimia, dan pengujian Klinik. Pedoman Pengujian dan Pengembangan Fitofarmaka. Pengembangan dan Pemanfaatan Obat Bahan Alam. Jakarta : Yayasan Pengembangan dan Pemanfaatan Obat Bahan Alam Ohyto Medica, 43-54.

Lelo A. dan Hidayat (2004). NSAIDS: Friend or Foe. Makassar: Journal of the Indonesia Dental Association.

Maimun, Z.A., Mudjiwijono, H.E., Arif, dan Muhammad Hamada. (2009). Efek Antikeradangan Ekstrak Daun Kemangi (Ocimum sanctum Linn) terhadap Jumlah Sel radang pada Plantar Pedis Rattus Norvegicus Jantan Strain Wistar yang diinduksi Karagenan. Majalah kedokteran, 1-9.

Quintans-Júnior, L.J., et al. (2011). Citral reduces nociceptive and infl ammatory response in rodents. Brazilian Journal of Pharmacognosy 21 (3), 497-502.

Sadraei, H., Ghannadi, A., and Malekshahi, K. (2003). Relaxant Effect of Essential Oil of Melissa officinalis and Citral on Rat Ileum contractions. Iran: Isfahan University of Medical Sciences. Fitoterapia 74, 445-452.

Sarma, S.D.K., and Babu, A.V.S. (2011). Pharmacognostic and Phytochemical Studies of Ocimum americanum. J. Chem. Pharm. Res 3(3), 331-347.

Schweitzer, A., Hasler-Nguyen, N., and Zijlstra, J. (2009). Preferential uptake of the non steroid anti-inflammatory drug diclofenac into inflamed tissues after a single oral dose in rats. BMC Pharmacology, 9:5 

Sherwood, L. (2001). Fisiologi Manusia Dari Sel ke Sistem. Edisi II. Jakarta: Penerbit Buku Kedokteran EGC, 369-379.

Siemonsma, J. S., Kasem, Piluek. (1994). Vegetaables. Prosea 8.

Silbernnagl, Stefan., and Lang, Florian. (2000). Color Atlas of Pathophysiology. USA: Thieme New York, 48-50.

Turk, Rhen, and Cidlowski, J.A. (2005). Mechanisms of Disease Antiinflammatory Action of Glucocorticoids. New Mechanisms for Old Drugs. The New England Journal of Medicine.

Umar, Anandini N.L. (2011). Perbandingan Ekstrak Daun Kemangi (Ocimum basilicum L.) dengan Ketokonazol 2% dalam menghambat Pertumbuhan Candida sp. Pada Kandidiasis Vulvovaginalis. Universitas Diponegoro: Fakultas Kedokteran.

Utami, Evy T., Kuncoro, Rebecca A., Hutami, Finsa T. S., dan Handajani, Juni. (2011). Efek Antiinflamasi Ekstrak Daun Sembukan (Paederia scandens) pada Tikus Wistar. Majalah Obat Tradisional 16(2), 95-100.

Vane, J.R., and Botting, R.M. (2008). Inhibition of prostaglandin synthesis’ as a mechanism of action for aspirin-like drugs. Dalam: Indonesian Journal of Dentistry 15(3), 200-204

Wilmana , P. F., dan Sulistia G.G. (2007). Analgesik-antipiretik, Analgesik-antiinflamasi non Steroid, dan Obat Pirai. Dalam: Sulistia G.G. (ed.). 2007. Farmakologi dan Terapi, ed 5. Jakarta: Bagian Farmakologi Fakultas Kedokteran Universitas Indonesia, 230-246, 500-506.