Vol 4, No 2 (2017) > Articles >

Uji Efektivitas Penyembuhan Luka Akut Stadium II Terbuka Kombinasi Fase Air-Minyak Ekstrak Ikan Gabus (Channa striata) Dalam Sediaan Salep Pada Tikus Jantan Galur Wistar

Mohamad Andrie , Dies Sihombing

 

Abstract:

The snakehead (Channa striata) extract contains albumin (in its water phase) and omega-3 and omega-6 (in its oil phase) which have been proven to be effective in wound healing process. This research was aimed to determine wound healing effect of snakehead extract-containing ointment on 6 groups of male Wistar rats. The rat's skin was wounded to produce acute stage II opened wound, the ointment was applied for sixteen days, and wound healing process was observed. The width of the wound was measured by using Macbiophotonic Image J program. The AUC results of each groups from smallest to largest were normal group (702.84% x day), negative group (749.56% x day), positive group (765.146% x day), water phase-containing ointment 10% group (795.146% x day), oil phase-containing ointment 10% (837.282% x day), and water-oil-phase combination ointment 10% (874.901% x day). The data were analyzed using One-Way ANOVA and Post-Hoc LSD test. The result of this research showed significant differences (p<0.05) between groups of water-oil phase combination of snakehead extract ointment with a negative control group. This result showed that ointment containing water-oil phase combination of snakehead extract had wound healing effect on acute stage II opened wound.


Keywords: snakehead extract; albumine; fatty acid; acute stage II open wounded healing; ekstrak ikan gabus; albumin; asam lemak; penyembuhan luka terbuka akut stadium II

Published at: Vol 4, No 2 (2017) pages: 88-101

DOI: 10.7454/psr.v4i2.3602


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References:

Andrie, M. (2015). Formulasi Salep Ekstrak Ikan Gabus (Channa striata) dengan Variasi Konsentrasi Basis. Penelitian Dosen dan Dipa. Program Studi Farmasi, Fakultas Kedokteran Universitas Tanjungpura.

Ardianto D. (2015). Buku Pintar Budi Daya Ikan Gabus. Yogyakarta: Flashbooks.

Astawan, M. (1998). Teknik Ekstraksi dan Pemanfaatan Minyak Ikan untuk Kesehatan. Buletin Teknologi dan Industri Pangan, 9(1), 44-51.

Calder, P.C. (2009). Polyunsaturated fatty acids and inflammatory processes: new twists in an old tale. Biochimie, 91(6), 791-795.

Collins, N., Sulewski, C. (2011). Omega-3 Fatty Acids and Wound Healing. Ostomy Wound Management; 10-13.

Daud, D.K.C., Mat Jais A.M., Ahmad, Z., Akim, A.M.D., Adam, A. (2010). Amino and fatty Acid Compositions In Haruan Traditional Extract (HTE). Bol. Latinom. Caribe. Plant. Med. Aromat, 9(5), 414-429.

Gadekar, R., Saurabh, M.K., Thakur, G.S., Saurabh, A. (2012). Studi of Formulation, Characterisation and Wound Healing Potential of Transdermal Patches of Curcumin. Asian Journal of Pharmaceutical and Clinical Research, 5(4), 225-230.

Gitaraja, W.S., Ekaputra, E. (2011). Metode Perawatan Luka. Dalam : Seminar Nasional Keperawatan, 13 November 2011. Jember : PSIK Universitas Jember

Gusdi, O. (2012). Formulasi Sediaan Gel Ekstrak Ikan Gabus (Channa striata) sebagai Obat Luka Sayat. Skripsi. Program Studi Farmasi, Fakultas Kedokteran, Universitas Tanjungpura Pontianak.

Hairima, Andrie, M., Fahruroji, A. (2014). Uji Aktivitas Salep Obat Luka Fase Air Ekstrak Ikan Toman (Channa micropeltes) pada Tikus Putih Jantan Galur Wistar. Jurnal Mahasiswa Farmasi Fakultas Kedokteran Universitas Tanjungpura, 1(1), 1-14.

Hankenson, K. D., Watkins, B.A., Schoenlein, I.A., Allen, K.G., Turek, J.J. (2000). Omega-3 Fatty Acids Enhance Ligament Fibroblast Collagen Formation In Association With Changes In Interleukin-6 Production. Proc. Soc. Exp. Biol. Med, 223(1), 88-95.

Kalbe Farma. (2009). BMK Penyembuhan Luka. FK UWKS 2012 C. [Doc]. Jul [dicitasi 05 Agustus 2016].

Kalbe Medical Portal. Bioplacenton. [Internet]. (2016). Dicitasi [05 Agustus 2016]. Tersedia dari: http://www.kalbemed.com/Products/Drugs/Branded/tabid/245/ID/5699/Bioplacenton.aspx

Levin, J., Miller, R.A. (2011). Guide To The Ingredients and Potential Benefits Of Over-The-Counter Cleansers And Moisturizers For Rosacea Patients. J Clin Aesthet Dermatol, 4(8). 31-49

Mat Jais, A.M. (2007) Pharmacognosy and Pharmacology of Haruan (Channa Striatus), A Medicinal Fish with Wound Healing Properties. Bol. Latinom. Caribe. Plant. Med. Aromat, 6(3), 52-60

Monteiro, et al. (2011). Leukotriene B4 Mediates Neutrophil Migration Induced by Heme. J Immunol, 186(11), 6562-6567.

Morton, J.J.P., Malone, M.H. (1992) Evaluation Of Vulnerary By An Open Wound Procedure in Rats. Archieve Int Pharmacodyn, 196, 117-128.

Mustafa, A., Widodo, M.A., Kristianto, Y. (2012). Albumin and Zinc Content Of Snakehead Fish (Channa striata) Extract and Its Role In Health. International Journal of Science and Technology (IJSTE), 1(2), 1-8.

Naveh, H.R., Jafari, Taghavi M.M., Shariati, M., Vazeirnejad, R., Rezvani M.E. (2011). Both Omega-3 and Omega-6 Polyunsaturated Fatty Acids Stimulate Foot wound Healing in Chronic Diabetic Rat. Afr J of Pharm and Pharmacol, 5(14),1713-1717.

Poedjiadi, A. (1994) Dasar – Dasar Biokimia. Jakarta: UI Press.

Rusjianto. (2009). Pengaruh Pemberian Suplemen Seng (Zn) dan Vitamin Terhadap Kecepatan Penyembuhan Luka Pasca Bedah di Rumah Sakit Umum Daerah Kabupaten Sukoharjo. Jurnal Kedokteran Indonesia, 1(1).

Sabale, P., Bhimani, B., Prajapati, C., Sabale, V. (2012). An Overview of Medicinal Plants as Wound Healers. Journal of Applied Pharmaceutical Science, 2(11), 143-150.

Serhan, C.N. (2007). Resolution Phase of Inflammation: Novel Endogenous Anti-Inflammatory and Proresolving Lipid Mediators and Pathways. Annu. Rev. Immunol, 25, 101–137.

Sinambela, H.Y. (2012). Optimasi Formulasi Sediaan Salep Minyak Ikan Gabus (Channa striata Bloch) sebagai Obat Luka Sayat dengan Metode Simplex Lattice Design. Skripsi. Program Studi Farmasi, Fakultas Kedokteran Universitas Tanjungpura Pontianak.

Suprayitno, E. (2009). Penggunaan Albumin Ikan Gabus (Ophiocephalus striatus) pada Penutupan Luka. Artikel Ilmiah, 1(2).

Taslim, N.A., Hidayanty, H., Jafar, N. (2007). Pengaruh Pemberian Kapsul Konsentrat Ikan Gabus pada Pasien Pasca Bedah di RSU DR. Wahidin Sudirohusodo Makassar. Bagian Gizi, Fakultas Kesehatan Masyarakat, Universitas Hasanuddin.

Voigt, R. (1994) Buku Pelajaran Teknologi Farmasi. Terjemahan Soendani Noerono Soewandhi. Yogyakarta: Gadjah Mada University Press.

Wathoni., Rusdiana, T., Hutagaol, R.Y. (2009). Formulasi Gel Antioksidan Ekstrak Rimpang Lengkuas (Alpinia galanga L.Willd) dengan Menggunakan Basis Aqupec 505 HV. Skripsi. Fakultas Farmasi Unuversitas Padjadjaran: Jatinangor.

Wijaya, U., Andrie, M., Fahrurroji, A. (2015). Uji Aktivitas Salep Fase Minyak Ekstrak Ikan Toman (Channa micropeltes) Terhadap Luka Sayat pada Tikus Jantan Galur Wistar. Jurnal Mahasiswa Farmasi Fakultas Kedokteran Universitas Tanjungpura, 3(1), 1-9.