Potensi Ekstrak Etanol Daun Petai (Parkia speciosa Hassk.) Terhadap Kadar Superoksida Dismutase (SOD) pada Plasma Tikus yang Mengalami Stres Oksidatif
Ruth Haryanti Butarbutar , Robiyanto Robiyanto , Eka Kartika UntariCigarette smoke is free radicals superoxide, hydrogen peroxide, hidrosil and peroksil. Superoxide dismutase (SOD) is an enzymatic antioxidants that protect cells from oxidative stress by catalyzing superoxide dismutase (O2*) into O2 and H2O2. This study aims to determine the potential of EELPs against plasma SOD enzyme activity in animals exposed by cigarette smoke. The simplicia extracted by maseration using ethanol 96%. Thirty-six rats Sprague Dawley strain were divided into six groups: normal group was not given treatment, negative group receiving 10% CMC-Na, positive control group receiving vitamin E, and treatment group received EELPs of 50; 100; 250 mg / kgBW of mice. All rats were treated for 14 days. SOD activity was measured by UV-Visible spectrophotometer at a wavelength of 505 nm. Statistical analysisusing One Way ANOVA. The mean value of SOD activity to the normal, negative control, positive control and the group receiving the EELPs dose of 50; 100; 250 mg/kg in a row is 37 979 ± 3708; 59 932 ± 3085; 48 552 ± 1234; 57 239 ± 2102; 50 774 ± 1632; 59 124 ± 4849. The results obtained show EELPs the most influence on the activity of SOD is a dose of 100 mg/kg.
Full PDF Download
References:
Aditama T.Y. (1992). Rokok dan Kesehatan. Jakarta : Univesitas Indonesia (3-5)
Departemen Kesehatan Republik Indonesia. (1995). Materia Medika Indonesia. Jilid IV. Jakarta: Departemen Kesehatan Republik Indonesia
Dewi, S., Budi, H., & Hendra, F. (2003). Penentuan Kadar Nikotin dalam Asap Rokok. Makara Kesehatan, 7(2), 38-41.
Makker K, Agarwal A, Sharma R.(2009). Oxidatuve stress and male infertility. Indian J Med Res. 129:357-67
Rami Al Batran, et al. (2013). In Vivo Antioxidant and Antiulcer Activity of Parkia speciosa Ethanolic Leaf Extract against Ethanol-Induced Gastric Ulcer In Rats. Malaysia: Faculty of Medicine Unibersity of Malaya.
SEATCA. (2014). The ASEAN Tobacco Control Atlas. Thailand : Southest Asia Tobacco Control Allience (SEATCA).
Sima Apriyana. (2015). Pengaruh Konsentrasi Penyari Etanol Ekstrak Daun Pare (Momordica Charantia L.) Terhadap Kadar Flavonoid Total.Skripsi. Universitas Hasanuddin.
Sumardika I Wayan, I Made Jawi. (2012). Ekstrak Air Daun Ubi Jalar Ungu Memperbaiki Profil Lipid dan Meningkatkan Kadar SOD Darah Tikus yang Diberi Makanan Tinggi Kolesterol. Jurnal Ilmiah Kedokteran.; 68-70
Tangkanakul Plernchai, et all. (2005). Extracts of Thai Indigenous Vegetables as Rancid Inhibitor in a Model System. Thailand: Kasetsart University.
Van der Vaart., Postma, D.S., Timens, w., & Ten, Hacken, N.H.T. (2004). Acute Effect of Cigarette Smoke on Inflammation and Oxidative Stress: a Review. Thorax, 59, 713-721
Yanbaeva, D.G., Dentener,M.A., Creutzberg, E.C., Wesseling, G., dan Wouters,E.F. (2007). Systemic Effect of Smoking . Chest ,131(5),1557-1566