Vol 3, No 3 (2016) > Articles >

Potensi Ekstrak Etanol Daun Petai (Parkia speciosa Hassk.) Terhadap Kadar Superoksida Dismutase (SOD) pada Plasma Tikus yang Mengalami Stres Oksidatif

Ruth Haryanti Butarbutar , Robiyanto Robiyanto , Eka Kartika Untari

 

Abstract:

Cigarette smoke is free radicals superoxide, hydrogen peroxide, hidrosil and peroksil. Superoxide dismutase (SOD) is an enzymatic antioxidants that protect cells from oxidative stress by catalyzing  superoxide dismutase (O2*) into O2 and H2O2. This study aims to determine the potential of EELPs against plasma SOD enzyme activity in animals exposed by cigarette smoke. The simplicia extracted by maseration using ethanol 96%. Thirty-six rats Sprague Dawley strain were divided into six groups:  normal group was not given treatment, negative group receiving 10% CMC-Na, positive control group receiving vitamin E, and treatment group received EELPs of 50; 100; 250 mg / kgBW of mice. All rats were treated for 14 days. SOD activity was measured by UV-Visible spectrophotometer at a wavelength of 505 nm. Statistical analysisusing One Way ANOVA. The mean value of SOD activity to the normal, negative control, positive control and the group receiving the EELPs dose of 50; 100; 250 mg/kg in a row is 37 979 ± 3708; 59 932 ± 3085; 48 552 ± 1234; 57 239 ± 2102; 50 774 ± 1632; 59 124 ± 4849. The results obtained show EELPs the most influence on the activity of SOD is a dose of 100 mg/kg.


Keywords: Parkia speciosa Hassk., superoksida dismutase, oxidative stress, cigarette smoke

Published at: Vol 3, No 3 (2016) pages: 97-106

DOI: 10.7454/psr.v3i3.3539


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