Vol 1, No 1 (2014) > Articles >

Formulasi Gel Pacar Air (Impatiens balsamina Linn.) terhadap Propionibacterium acnes dan Staphylococcus epidermidis

Diah Ismarani , Liza Pratiwi , Indri Kusharyanti

 

Abstract:

Acnes vulgaris is a chronic inflammatory disease of sebaceous follicles caused by Propionibacterium acnes and Staphylococcus epidermidis. The antibacterial activity of Impatiens balsamina L. has been studied for many years. The aims of this study are to find out the antiacne effect from methanolic extract of stems and leaves of Impatiens balsamina L. that formulated in gel. Simplicia were extracted using soxhlet technique bu using methanol as solvent. Extracts were then formulated in gel in three variations  of HPMC 4000 and Carbopol 934 as gel base with ratio of 70:30 (F1), 50:50 (F2) and 30:70 (F3). The determination of antiacne effect was done using disc diffusion method. Evaluation of physical and chemical properties of those gels includes organoleptic examination, spreadability testing, adhesion testing and pH testing. Data were analyzed using R version 2.14.1 package R-commander. Determination results showed the diameter of inhibition zone from FI, FII and FIII for P.acnes are 6.46 ±0.15 mm; 12.16 ± 0.35 mm; 16.13 ± 0.35 mm and for S. epidermidis sebesar 14.5 ± 0.47 mm; 16.56 ± 0.651 mm; 17.13 ± 0.44 mm. Analysis results showed that gel of FIII gave optimal antiacne effect, and significantly different from positive control (p<0,05). The evaluation of gel showed that they have homogeny texture, good spreadability and adhesion, as well as the pH.


Keywords: Impatiens balsamina Linn.; Propionibacterium acnes; Staphylococcus epidermidis

Published at: Vol 1, No 1 (2014) pages: 30-45

DOI: 10.7454/psr.v1i1.3504


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References:

Adfa, M. (2007). Senyawa Antibakteri dari Daun Pacar Air (Impatiens balsamina L). Jurnal Gradien, 4(1), 318-322

Anggraini., Deni., Malik, M., Susiladewi, M. (2006). Formulasi Krim Serbuk Getah Buah Pepaya (Carica papaya L) Sebagai Anti Jerawat. Universitas Wahid Hasyim Publikasi Ilmiah. Semarang.

Clinical and Laboratory Standards Institute. (2006). Performance standards for antimicrobial disk susceptibility tests. Approved standard M2-A9. CLSI, Wayne, PA.

Cowan, M.M. (1999). Plant Produces as Antimicrobial Agents. Clinical Microbiology Reviews, 12 (4), 564-582

Cushnie, T.P., Lamb, A.J.(2005). Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents, 26(5), 343-356

Danester Quiñones, ms; evones. Ghaly, Phd. (2008). Formulation and characterization of nystatin gel. Puerto Rico Health Sciences Journal, 27(1), 61-67

Departemen Kesehatan Republik Indonesia. .(1995). Farmakope Indonesia (edisi keempat). Jakarta: Direktorat Jenderal Pengawasan Obat dan Makanan.

Direktorat Jenderal Pengawasan Obat dan Makanan. (1995). Materia Medika Indonesia. Jilid III. Departemen Kesehatan RI. Jakarta

Novy, F.O. (2013). Uji Efektivitas Sediaan Gel Antijerawat Ekstrak Etanol Rimpang Jahe Merah (Zingiber officinale Rosc.) Terhadap P. acnes dan S. epidermidis. Skripsi. Pontianak:Fakultas Kedokteran Universitas Tanjungpura

Nurdin, G.M., Dirayah., Sartini. (2009). Bioaktifitas Ekstrak Metanol Daun Pacar Air (Impatient balsamina Linn.) terhadap Pertumbuhan Bakteri Staphylococcus aureus dan Pseudomonas aeruginosa Penyebab Cantengan. Makassar: Universitas Hasanuddin.

Kang, S.N., Goo, Y.M., Yang, M.R., Ibrahim, R., Cho, J.H., Kim, I.S., et al. (2013). Antioxidant and antimicrobial activities of ethanol extract from the stem and leaf of Impatiens balsamina L. (Balsaminaceae) at different harvest times. Molecules, 18(6), 6356-6365

Lim, Y.H., Kim, I.H., Seo, J.J. (2007). In vitro Activity of Kaempferol Isolated from the Impatiens balsamina alone and in Combination with Erythromycin or Clindamycin against P. acnes. The Journal of Microbiology, 45(5), 473-477.

Ohemeng, K.A., Schwender, C.F., Fu, K.P., Barrett, J.F. (1993). DNA gyrase inhibitory and antibacterial activity of some flavones (1). Bioorganic & Medicinal Chemistry Letters, 3(2), 225–30

Panichayupakaranant, P. (2001). Napthoquinone Formation in Impatiens balsamina Cell Cultures. Pharmaceutical Biology, 39(4), 293-296

Sakunphueak, A., Panichayupakaranant, P. (2012). Comparison of antimicrobial activities of naphtaquinones from Impatiens balsamina. Natural Product Research, 26(12),1119-1124

Su, B.L., Zeng, R., Chen, C.Y., Guo, J.H., Huang, C.G. (2012). Antioxidant and antimicrobial properties of various solvemt extract from Impatiens balsamina L. Stems. Journal of Food Sciences, 77(6), 614-619

Suardi, M., Armenia, Anita, M. (2008). Formulasi dan Uji Klinik Gel Anti Jerawat Benzoil Peroksida-HPMC. Jurnal Sains dan Teknologi Farmasi.

Sudjono, T.A., Honniasih, M., Pratimasari, Y.T. (2012). Pengaruh Konsentrasi Gelling Agent Carbomer 934 dan HPMC Pada Formulasi Gel Lendir Bekicot (Achatina fulica) Terhadap Kecepatan Penyembuhan Luka Bakar Pada Punggung Kelinci. PHARMACON, 13(1), 6-11

Utari, P. (2011). Skrining Fitokimia dan Uji Aktifitas antibakteri ekstrak daun dari tumbuhan pacar air (Impatient balsamina Linn.) terhadap bakteri Staphylococcus aureus, S. epidermidis dan Pseudomonas auruginosa . Skripsi. Medan : Universitas Sumatera Utara

Wang, Y.C., Li, W.Y., Wu, D.C., Wang, J.J., Wu, C.H., Liao, J.J., et al. (2009). In Vitro Activity of 2-methoxy-1,4-naphtaquinon and Stigmasta-7,22 diene-3b-ol from Impatiens balsamina L. Against Multiple Antibiotic-Resistant Helicobacter pylory. Hindawi Publishing Coorporation.

Wasitaatmadja, S.M. (1997). Penuntun Ilmu Kosmetik Medik. Jakarta: UI-Press

Vats, A., Sharma, P. (2012). Formulation and Evaluation of Topical Anti Acne Formulation of Coriander Oil. American Journal of PharmaTech Research, 2(5), 452-464