Vol 1, No 2 (2014) > Articles >

Potensi Pemanfaatan Nigella sativa L. sebagai Imunomodulator dan Antiinflamasi

Farida Sulistiawati , Maksum Radji



Nigella sativa L. has long been used as an herbal medicine in some countries, particularly in the Middle East and in other Asian countries, including in Indonesia. One component is a protein compound extracted from black cumin oil residues, known to have efficacy to enhance the immune system and as an anti-inflammatory. As the compound will be degraded when administered orally, the research on its delivery systems, is interesting to be developed. This article will review about the prospects of their utilization and strategy formulation that preparation can be delivered orally.

Keywords: Nigella sativa L., protein, imunomodulator, partikelnano

Published at: Vol 1, No 2 (2014) pages: 65-77

DOI: 10.7454/psr.v1i2.3493

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Adamu, H.M., et. al. (2010). Identification of Essential Oil Components from Nigella sativa Seed by Gas Chromatography Mass Spectroscopy. Pakistan Journal of Nutrition, 9(10), 966-967.

Al-Beitawi, N., El-Ghousein, S.S.(2008). Effect of feeding Different Levels of Nigella sativa L. Sedds (Black cumin) on Performance Blood Constituent and Carcass Charactristics of broiler Chicks. International Journal of Poultry Science, 7, 715-721.

AlGhamdi, M.S. (2001). The Anti Inflammatory Analgesic and Antipyretic Activity of Nigella sativa. Journal Ethnopharmacol, 76, 45-48.

Al-Naqeep, G.N., et al. (2009). Nutrients Composition and Minerals Content of Three Different Samples of Nigella sativa L. Cultivated in Yemen. Asian Journal of Biological Sciences, 2, 43-48.

Ali, A.A. (2008). Oral and Intraperitoneal LD50 of Thymoquinone an Active Principle of Nigella sativa in Mice and Rats. Journal of Ayub Medical College Abbottabad, 20(2), 25-27.

Bai, J.P.F., Amidon, G.L. (1992). Structural Specificity of Mucosal-Cell Transport and Metabolismof Peptide Drugs: Implication for Oral Peptide Drug Delivery. Pharmaceutical Research, 9, 969–978.

Bernkop-Schnurch, A., Clausen, A.E. (2002). Bio membrane permeability of peptides: Strategies to improve their mucosal uptake. Mini Review in Medicinal Chemistry, 2, 295–305.

Boskabady, M.H., et al. (2011). Potential Immunomodulation effect of The Extract of Nigella sativa on Ovalbumin Sensitized Guinea Pigs. Biomed and Biotechnology, 12(3), 201–209.

El-Bagir, N.M., et al. (2006). Lipid Composition of Egg Yolk and Serum in Laying Hens Fed Diets Containing Black Cumin (Nigella sativa). International Journal of Poultry Science, 5, 574-578.

El-Gazzar, M., et al. (2006). Anti Inflammatory Effect of Thymoquinone in a Mouse Model of Allergic Lung Inflammation. International Immunopharmacology, 6(7), 1135-1142.

El Kadi, M., Kandil, O., Tabuni, A.M. (1990). Nigella sativa and Cell Mediated Immunity. Arch Aids Res, 1, 232-235.

El-Mahmoudy, A., et al. (2002). Thymoquinone supresses Expression of inducible Nitric Oxide Synthase in rat Macrophages. International Immunopharmacology, 2(11), 1603-1611.

El-Nattat, W., El-Kady, R.(2007). Effect of Different Medicinal Plant Seeds Residues on The Nutritional and Reproductive Performance of Adult Male Rabbits. International Journal of Agricultural and Biological, 9, 479-485.

Ghonime, M., et al. (2011). Evaluation of Immunomodulatory Effect of Three Herbal Plants Growing in Egypt. Immunopharmacol Immunotoxicol, 33(1), 141-145.

Hailat, N., et al. (1998). Effects of Nigella sativa Extract on Antibody response of Rats Vaccinated with Brucella Vaccine (Rev-1).Pharmaceutical Biology, 36(3), 217-221.

Hamman, J.H.,et al. (2005). Oral delivery of peptide drugs. Bio Drugs, 19, 165–177.

Haq, A., et al. (1995). Nigella sativa: Effect on Human Lymphoctes and Polymorphonuclear Leukocyte Phagocytic Activity. Immunopharmacology, 30(2), 147-155.

Haq, A.(1996). Fractination of Black Seed (Nigella sativa L.) Protein by using Rotofor. Journal of Liquid Chromatography & Related Technologies, 19(4), 593-599.

Haq, A., Lobo, P.I., Al-Tufail, M., Rama N.R., Al-Sedairy, S.T. (1999). Immunomodulatory Effect of Nigella sativa protein Fractionated by Ion Exchange Chromatography. International Journal of Immunopharmacology, 21(4), 283-295.

Hussain, N. (2000). Ligand-mediated tissue specific drug delivery, Adv. Drug Delivery Reviews, 43, 95–100.

Holman, R.T. (1986). Control of polyunsaturated acids in tissue lipids. The Journal of the American College of Nutrition, 5, 183–211.

Hussain, N. (2000). Ligand-mediated tissue specific drug delivery. Advanced DrugDelivery Reviews,43, 95–100.

Islam, S.K.N., et al. (2004). Immunosuppresive and Cytotoxic Properties of Nigella sativa. Phytotherapy Research, 18(5), 395-398.

Junginger, H.E. (1990). Bioadhesive polymer systems for peptide delivery. Acta Pharmaceutical. Technology, 36, 110–126.

Jahanshahi, M., Zhang, Z., Lyddiatt, A. (2005). Subtractive chromatography for purification and recovery of nano-bioproducts.IEE ProcNanobiotechnol, 152(3), 121–126.

Khader, M., et al. (2009). In Vivo Toxicological Properties of Thymoquinone. Food and Chemical Toxicology, 47(1), 129-33.

Khasanah, N. (2009). Pengaruh Pemberian Ekstrak Nigella sativa terhadap Respon Proliferasi Limfosit Limfa Mencit Balb/C yang diinfeksi S. Typhimurium. Fakultas Kedokteran, Universitas Diponegoro, Semarang.

Mahato, R.I.,et al. (2003). Emerging trends in oral delivery of peptide and proteins.Critical Reviews in Therapeutic Drug Carrier Systems, 20, 153–214.

Manu, K.A., Kuttan, G.(2009). Immunomodulatory Activities of Punarnavine an Alkaloid from Boerhaavia diffusa. Immunopharmacol Immunotoxicol, 31(3), 377-873.

Mahmood, M.S., et al. (2003). The In Vitro Effect of Aqueous Extract of Nigella sativa Seeds on Nitric Oxide Production. Phytotherapy Research, 17(8), 921-924.

Majdalawiech, A.F., (2010). Nigella sativa Modulates Splenocyte Proliferation, Th1/Th2 Cytokine Profile macrophage Function and NK Anti Tumor Activity. Journal of Ethnopharmacology, 131(2), 268-275.

Michel, C.G., et al. (2010). Phytochemical and Biological Investigation of The Extract of Nigella sativa L.seed Waste. Drug Testing and Analysis, 3(4), 245-254.

Morishita, M., Peppas, N.A. (2006). Is the oral route possible for peptide andprotein drug delivery. Drug Discovery Today, 11, 905-910.

Mohanraj, V., Chen, Y. (2006). Nanoparticles a review. Tropical Journal of Pharmaceutical Research, 5, 561–573.

Nickavar, B., et al. (2003). Chemical Composition of The Fixed and Volatile Oils of Nigella sativa L. from Iran. Z. Naturforsch C, 58, 629-631.

Pisal, D.S., Kosloski, M.P., Balu-Iyer, S.V. (2010). Delivery of therapeutic protein. Journal of Pharmaceutical Sciences, 99(6), 2557–2575.

Rahimnejad, M., Mokhtarian, N., and Ghasemi, M. (2009). Production of protein nanoparticles for food and drug delivery system. African Journal of Biotechnology, 8(19), 4738-4743.

Ram, I.M., Ajit, S.N., Laura, T., Duane, D.M. (2003). Emerging trends in oral delivery of peptide and protein drugs. Critical Reviews in Therapeutic Drug Carrier Systems, 20, 153–214.

Sari, A.I.P.(2009). Pengaruh Pemberian ekstrak Nigella sativa terhadap Produksi NO Makrofag Mencit Balb/C yang diinduksi Salmonella typhimurium. Fakultas Kedokteran, Universitas Diponegoro, Semarang.

Shaji, J., Patole. (2008). Protein and Peptide Drug Delivery: Oral Approaches. Indian Journal Pharmaceutical Sciences, 70(3), 269–277.

Suhatri., Aldi, Y. (2010). Aktifitas Ekstrak Etanol Nigella sativa terhadap Titer Antibodi dan Jumlah Sel Leukosit pada Mencit Putih Jantan. Scientia, 1(1), 35-41.

Sarker, M.R.(2011).In Vitro Enhancement of Polyclonal IgM Production by Ethanolic Extract of NS Seeds in Whole Spleen Cells of Female Balb/C Mice. Bangladesh Pharmaceutical Journal,14(1), 73-77.

Silvia, D., Farah Masturah M., Tajul Aris Y., Wan Nadiah, W.A., Bhat, R. (2012). The Effects of Different Extraction Temperatures of the Screw Press on Proximate Compositions, Amino Acid Contents and Mineral Contents of Nigella sativa Meal. American Journal Food Technology, 7(4), 180-191.

Soppimath, K.S., Aminabhavi, T.M., Kulkarni, A.R., Rudzinski, W.E. (2001). Biodegradable polymeric nanoparticles as drug delivery devices. Journal Control Release, 70, 1–20.

Swamy, S.M.K., and Tan, B.K.H.(2000). Cytotoxic and Immunopotentiating Effects of Ethanolic Extract of Nigella sativa Seeds. Journal Ethnopharmacology, 70, 1-7.

Tasawar, Z., et. al. (2011). The Effects of Nigella sativa (Kalonji) on Lipid Profile in patients with Stable Coronary Artery Disease in Multan, Pakistan. Pakistan Journal of Nutrition, 10, 162-167.

Tauseef, S.M., et al.(2009). Safety Assessment of Black Cumin Fixed and Essential Oilin Normal Sprague Dewley rats: Serological and hematological Indices. Food Chemistry Toxicology, 47(11), 2768-2775.

Vahdati, M.N., et al. (2005). An Investigation on LD50 and Sub Acute Hepatic Toxicity of Nigella sativa Sedds Extract in Mice.Pharmazie, 60(7), 544-547.

Wu, D., et al.(1999). Effect of Dietary Supplementation with Black Current Seed Oil on The Immune Respone of Healthy Elderly subjects. The American Journal of Clinical Nutrition, 70(4), 536-543.

Woodley, J.F. (1994). Enzymatic barriers for GI peptide and protein delivery. Critical Reviews in Therapeutic Drug Carrier Systems,11, 61–95.

Yun, Y., Cho, Y.W., Park, K. (2013). Nanoparticles for oral delivery: Targeted nanoparticleswith peptidic ligands for oral protein delivery. Advanced Drug Delivery Reviews, 65(6), 822–832.

Zaoui, A.,et al. (2002). Acute and Chronic Toxicity of Nigella sativa Fixed oil. Phytomedicine, 9(1), 69-74.

Zeweill, H.S., et al. (2008). Evaluation of Substituting Nigella Seed Meal as Source of protein for Soybean Meal in Diets of New Zealand White Rabbits.Nutrition Digestive Physiology,863-867.