Vol 1, No 3 (2014) > Articles >

Efek Fraksi n-Heksana Kulit Hylocereus polyrhizus Terhadap Kadar Malondialdehida Tikus Stres Oksidatif

Wirna Maya Sari , Sri Wahdaningsih , Eka Kartika Untari

 

Abstract:

Excessive free radical production will lead to oxidative stress. One of the indicators used to determine stress oxidative is malondialdehyde (MDA). Hylocereus polyrhizus peel is potential to be used as a natural exogenous antioxidant. The aim of this research was to study the effect of n-hexane fraction of H. polyrhizus peel on MDA level and to determine the dose that could reduce the MDA level. H. polyrhizus peel was macerated with chloroform and fractionated with n-hexane. Rats were divided into 7 groups: normal control, negative control, positive control of vitamin E (18 mg/ kg BW), positive control of quercetin (4 mg/200g BW), as well as three H. polyrhizus peel extract groups treated with dose I (10mg/ 200g BW), dose II (20 mg/200g BW) and dose III (40 mg/200g BW). Each group was consisted of 4 rats and was induced to oxidative stress with 10 minute swimming each day and food restriction for 5 days. The measurement of MDA level used Spectrophotometer UV-Vis at the wavelenght of 532 nm. MDA levels were analyzed using One way Anova and LSD test. The data showed that MDA level at normal, negative, positive vitamin E, positive quercetin, dose I, II, III group were 0.042; 0.051; 0.034; 0.042; 0.038; 0.039; 0.042 µg/ml, respectively. The n-hexane fraction of H. polyrhizus peel was a potent antioxidant because it could lower the MDA level in rats with stress oxidative conditon.


 


Keywords: stress oxidative; malondialdehyde; antioxidant; Hylocereus polyrhizus; n-hexane; stres oksidatif; malondialdehida; antioksidan; n-heksana

Published at: Vol 1, No 3 (2014) pages: 154-165

DOI: 10.7454/psr.v1i3.3487


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References:

Budilaksono, W. (2013). Uji Aktivitas Antioksidan Fraksi n-Heksana Kulit Buah Naga Merah (Hylocereus Lemairei Britton Dan Rose) Menggunakan Metode DPPH (1,1-Difenil-2-Pikrilhidrazil). Skripsi. Pontianak: Program Studi Farmasi, Universitas Tanjungpura.

Burton, G.W., & Ingold, K.U. (1984). Beta Carotene: An Unusual Type Of Lipid Antioxidant. Science, 224, 569–573.

Chevion, S., Moran, D.S., Heled, Y., Shani, Y., Regev, G., Abbou, B., Berenshtein, E., et al. (2003). Plasma antioxidant status and cell injury after severe physical exercise. Proceedings of the National Academy of Sciences, 10051, 19–23

Departemen Kesehatan Republik Indonesia. (1979). Farmakope Indonesia. (3rd ed). Jakarta: Departemen Kesehatan Republik Indonesia.

Foti, Mc., & Ingold, Ku. (2003). Mechanism of Inhibition of Lipid Peroxidation By Gamma-Terpinene, An Unusual And Potentially Useful Hydrocarbon Antioxidant. Journal of Agricultural and Food Chemistry, 51(9), 2758-65.

Guyton, C.A. (1997). Buku Teks Fisiologi Kedokteran. Jakarta: EGC penerbit buku kedokteran.

Harjanto. (2005). Pemulihan Stres Oksidatif Pada Latihan Olahraga . Jurnal

Kedokteran YARSI, 12(3), 81-87.

Isabelle, M., Bee, L.L., Meng, T.L., Woon-Puay, Koh., Dejian, H., Choo, N.O. (2010). Antioxidant activity and profiles of common fruits in Singapore. Journal Food Chemistry, 123, 77–84.

Jang, Yoon Young., Song, Jin Ho., Shin, Yong Kyoo., Han, Euk Soon., Lee, Chung Soo. (2000). Protective Effect Of Boldine On Oxidative Mitochondrial Damage In Streptozotocin-Induced Diabetic Rats. Pharmacological Research, 42(4), 361- 371.

Jang, Moon Hee., Kim, Hyun Young., Kang, Ki Sung., Yokozawa, T., Park, Jeong Hill. (2009). Hydroxyl Radical Scavenging Activities Of Isoquinoline Alkaloids Isolated From Coptis Chinensis. Archives of Pharmacal Research, 32(3), 341-345.

Jawi, I., Manuaba, I., Sutirtayasa, I., & Muruti, G., (2006). Pemberian Glutamin Menurunkan Kadar Bilirubin Darah serta Mengurangi Nekrosis Sel-Sel Hati setelah Pemberian Aktivitas Fisik Maksimal dan Parasetamol pada Mencit. Dexa Media, No. 4, vol 19, 192-195.

Kikuzaki, H., Hisamoto, M., Hirose, K., Akiyama, K., & Taniguchi, H. (2002) Antioxidants Properties of Ferulic Acid and Its Related Compound. Journal of Agricultural and Food Chemistry, 50:2161; 50 (7), 2161-2168.

Komnas Lansia Penyakit Degeneratif. (2010). Warta Kesra edisi 121/15-30 website: http://www.komnaslansia.go.id/ modules.php?name=News&file=article &sid=50

Kurniasari, B., Aulanni’am & Anna Roosdiana. (2014). Pengaruh Herbal Spray Berbasis Bioaktif Spirulina Sp. Terhadap Kadar Mda Pada Luka Sayatan Tikus (Rattus Norvegicus) Dm T1. Kimia Student Journal, 1(1), 126-132

Moura. (2007). Antioxidant Properties Of B-Carboline Alkaloids Are Related To Their Antimutagenic And Antigenotoxic Activities Mutagenesis. Advance Access Publication, Vol. 22 No. 4 .

Nawar, W. (1985). Lipids. Didalam : fennema, O.R (ed). Food Chemistry. New York: Marcel Dekker Inc – Basel

Nayanatara, A.K., Nagaraja, H.S., & Anupama, B.K. (2005). The Effect Of Repeated Swimming Stress On Organ Weights And Lipid Peroxidation In Rats. Thai Journal Of Physiological Sciences, Vol 18, No.1.

O’Donnel., Chumley., Hogg., Bloodsworth, A., Darley, V.M &Freeman, B.A. (1997). Nitric Oxide Inhibition of Lipid Peroxidation: Kinetics of Reactions with Lipid Peroxyl Radicals and Comparison with Tocopherol. Biochemistry, 15216-15223.

Orellana, M., Fuentes, O., Rosenbluth, H., Lara, M., & Valdes, F. (1992). Modulatios of rats liver peroxisomal and microsomal fatty acids oxidation by starvation. FEBS, 310, 193-196.

Prasetyawati, R.C. (2003). Evaluasi Daya Antioksidatif Oleoresin Jahe (Zingiber officinale) Terhadap Aktivitas Superoksida Dismutase (SOD) Hati Tikus yang Mengalami Perlakuan Stres. Skripsi. Bogor: Fakultas Teknologi Pertanian Bogor.

Pokorny, J., Yanishlieva, H., & Gordon, M. (2001). Antioxidant In Food: Practical Application. Cambrige England: Woodhead

Rodriguez, M.C., Rosenfeld, J., & Tarnopolsky, M.A. (2003). Plasma Malondialdehyde Increases Transiently Ischemic Forearm Exercise. Medicine & Science in Sports & Exercise, 35(11), 1859, 65.

Puspawati, G.A. (2009). Kajian Aktivitas Proliferasi Limfosit dan Kapasitas Antioksidan Sorgum (Sorghum Bicolor L Moench) dan Jewawut (Pennisetum Sp) Pada Tikus Sprague Dawley. Tesis. Bogor: Institut Pertanian Bogor.

Singh, V.N. (1992). A Current Perspective on Nutrition and Exercise. The Journal Of Nutrision, 0022-3166/92

Singh, R.P, Murthy, K.N.C., & Jayaprakasha, G.K., (2002). Studies on Antioxidant Activity of Pomegranate (Punica granatum) Peel and Seed Extract Using in vitro Model. Journal of Agricultural and Food Chemistry, 50,

-86.

Suarsana., Wresditati, T., & Suprayogi. (2013). Respon Stres Oksidatif dan Pemberian Isoflavon terhadap Aktivitas Enzim Superoksida Dismutase dan Peroksidasi Lipid pada Hati Tikus. Jurnal Univ. Udayana. JITV, 18(2), 416-152.

Voigt, R. (1995). Buku Pelajaran Teknologi Farmasi. Yogyakarta : Gadjah Mada University Press.

Winarsi, H. (2007). Antioksidan Alami dan Radikal Bebas: Potensi dan Aplikasinya dalam Kesehatan. Yogyakarta: Kanisius.

Wresdiyati, Made, A., Diini., I Ketut., Savitri, N., & Saptina. (2002). Pengaruh Α-Tokoferol Terhadap Profil Superoksida Dismutase dan Malondialdehida Pada Jaringan Hati Tikus Di Bawah Kondisi Stres. Jurnal Veteriner, 13, 111

Wresdiyati, T., & Makita, T. (1995). Remarkable increase of peroxisomes in the renal tubule cells of Japanese monkeys under fasting stress. Pathophysiol, 2, 177-182.

Yagi, K. (1994). Free Radical in Diagnostic Medicine. Armstrong D, editor. New York: Plenum Pr.