Vol 2, No 1 (2015) > Articles >

Penetapan Kadar Fenolik dan Flavonoid Total Ekstrak Metanol Buah dan Daun Patikala (Etlingera elatior (Jack) R.M.SM)

Aktsar Roskiana Ahmad , Juwita Juwita , Siti Afrianty Daniya Ratulangi , Abdul Malik

 

Abstract:

Fruit and leaves of patikala (Etlingera elatior (Jack) R.M.Sm), is a traditional medicinal plants used by people to treat various diseases. The purpose of this study was to determine the total phenolic and flavonoid compounds of    E. elatior fruits and leaves extracts.    We extracted dried fruits and leaves samples by maceration method using methanol. Qualitative analysis of chemical compounds with certain eluent was done using Thin Layer Chromatography (TLC) to determine the group of active compounds in the extract. The amount of phenolic compounds in the sample was determined by the colorimetric method at a maximum wavelength of 744.8 nm and flavonoid at 431 nm. The results showed that the methanol extract rendemen was 1.93% for fruit and 5.17% for leaf with a total phenolic content of the fruit was 2.29 mgGAE/g b/b extract and leaf was 6.29 mgGAE/g b/b extract. And total flavonoid content of the fruit was 1.7761 % mg Quercetin/g b/b extract and leaf was 5.45 mgQE/g b/b extract.


Keywords: colorimetric method, patikala (Etlingera elatior (Jack) R.M.Sm), phenol, flavonoid.

Published at: Vol 2, No 1 (2015) pages: 1-10

DOI: 10.7454/psr.v2i1.3481


Access Counter: 3936 views

Full PDF Download

References:

Andayani, R. 2008. Penentuan Aktivitas Antioksidan Kadar Fenolat Total Dan Likopen Pada Buah Tomat (Solanamum lycopersicum L.) Fakultas Farmasi Universitas Andalas : Padang.

Antoro, E.D. 1995. Skrining Fitokimia Rimpang Nicolaia speciosa Horan Secara Mikrokimiawi Kromatografi Lapis Tipis, dan Spektrofotometri UV. FF-UGM : Bandung.

Apsari, Pramudita Dwi., & Susanti, H. 2011. Penetapan kadar fenolik total ekstrak Metanol kelopak bunga rosella merah (Hibiscus sabdariffa Linn) dengan variasi tempat Tumbuh secara spektrofotometri, 2, 1, 73-80.

Chun, O.K., Kim D.O., and Lee C.Y. 2003. Superoxide Radical Scavenging Activity of the Mayor Polyphenols in Fresh Plums, J Agric. Food Chem., i51:8067-8072.

Hakim, A.R. 2009. Uji Potensi Antifungi Ekstrak Etanol Rimpang Kecombrang (Nicolaia speciosa Horan) Terhadap Trichophyton mentagrophytes dan Trichophyton rubrum. [skripsi]. Program Studi Farmasi.UIN Syarif Hidayatullah : Jakarta.

Harborne, J.B. 1987. Metode Fitokimia : Penuntun cara modern menganalisa tumbuhan. Terbitan Kedua. Terjemahan Kosasih Padmawinata dan Iwang Soediro. ITB : Bandung.

Haris, A.R. 2009. Uji Potensi Antifungi Ekstrak Etanol Rimpang Kecombrang (Niolaia speiosa Horan) Terhadap Trichophyton mentagrophytes dan Trichophyton rubrum. (Skripsi). Program Studi Farmasi.UIN Syarif Hidayatullah: Jakarta.

Krismawati, A. 2007. Uji Toksisitas Beberapa Jenis Tanaman Indonesia Yang Dipercaya Dapat Menurunkan Berat Badan (Ceremai, Jati Belanda, Kunci Pepet, Delima Putih, Bangle,Kemuning) Terhadap Proliferasi Sel limfosit Manusia Secara In Vitro. Skripsi. IPB : Bogor.

Kurniasari, I. 2006. Metode Cepat Penentuan Flavanoid Total Meniran (Phyllantus niruri L) Berbasis Teknik Spektrofotometri Inframerah Dan Kemometrik. IPB: Bogor.

Miller, A.L. 1996. Antioxidant flavonoids: structure, function, and clinical usage. Alt Med Rev1:103 – 111

Naufalin, Rifda. 2005. Kajian Sifat Antimikroba Ekstrak Bunga Kecombrang (Nicolaia speciosa Horan) Terhadap Berbagai Mikroba Patogen dan Perusak Pangan. (Tesis) Jurusan Tekhnologi Pangan, Fakultas Pertanian. IPB: Bogor.

Primadini, R.,D. 2010. Uji Aktivitas Pengkhelatan Besi Pada Ekstrak Metanol Tanaman Obat Pegagan (Centella asiatika), Bunga Merak (Caesalpinia pulcherimma) dan Sendilaw Udang (Commersonia batramia) (Skripsi). Universitas Bengkulu: Bengkulu.

Raden Adityo HPP, Betta Kurniawan, Syazili Mustofa. 2012. Uji Efek Fraksi Metanol Ekstrak Batang Kecombrang (Etlingera elatior) Sebagai Larvasida Terhadap Larva Instar III Aedes aegypti. MAJORITY (Medical Journal of Lampung University).

Ukieyanna, E. 2012. Aktivitas Antioksidan, Kadar Fenolik, dan Flavanoid Total Tumbuhan Suruhan (Peperomia pellucid L. Kunth). Bogor : Fakultas Teknologi Pertanian Institut Pertanian Bogor.

Valianty K. 2002. Potensi Antibakteri Minyak Bunga Kecombrang [skripsi]. Jurusan Tekhnologi Pertanian, Fakultas Pertanian. Universitas Jendral Soedirman : Purwokerto.