Vol 7, No 3 (2010) > Articles >

Analisis Asam Valproat dalam Plasma Secara Kromatografi Gas

Ani Susanti , Yahdiana Harahap , Harmita Harmita



Valproic acid is an anticonvulsant drug that works by increasing the levels of
γ-aminobutyric acid (GABA). Determination of valproic acid is quite difficult
because it has no chromophore groups in its structure. Aa analytical method using
gas chromatography (GC) with flame ionization detector for the determination of
valproic acid in human plasma has been developed and optimized. Valproic acid was
extracted from plasma by liquid-liquid extraction method using diethyl ether. The
optimum analysis conditions for valproic acid in plasma were achieved by regulated
gas chromatography injector and detector at a temperature of 250oC and temperature
programming with an initial temperature of 70oC and 5oC temperature increasing
per minute until a temperature of 100oC, then held for 1 minute. Then the temperature
was increased by 2°C per minute until the column temperature to 150oC. The
optimum conditions of analysis took 32 minutes. In the concentration range from
40.0 to 100.0 µg/mL yielded a linear calibration curve with correlation coefficient (r)
of 0.9894. Accuracy (% diff) of this method was -13.67% to 12.33% with precision
(CV) between 9.33% to 14.92%, and relative recovery test was 86.33% to 112.33%.

Keywords : gas chromatography, optimation, plasma in vitro, valproic acid

Published at: Vol 7, No 3 (2010) pages: 32-45

DOI: 10.7454/psr.v7i3.3459

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