Vol 9, No 1 (2012) > Articles >

Efek Antidiabetes dan Identifikasi Senyawa Dominan Fraksi Kloroform Herba Ciplukan (Physalis angulata L.)

. Sediarso , Hadi Sunaryo , Nurul Amalia



Antidiabetic activity of ciplukan (Physalis angulata L.) herb has been widely observed,

as an extracts and infusions, proven to reduce blood sugar levels in rats or mice induced

by alloxan. The research objective was to evaluate the antidiabetic effect and identify the

content of the chloroform fraction ciplukan herb eluated with methanol-ammonia. An-

tidiabetic effects testing conducted using 30 male white mice (Mus musculus) ddY strain

were divided into 6 groups, each consisting of 5 mice. K-1 is a negative control induced by

alloxan, K-2, K-3 and K-4 induced by alloxan and treated with the test substance dose of

50 mg/kg bw/day, 100 mg/ kg bw/day, 200 mg/kg bw/day, K-5 is a positive control, induced

by alloxan and treated with metformin dose of 65 mg/kg bw, K-6 as normal controls . Each

mouse blood samples taken on day 7 and day 14, to test their blood sugar levels. Data were

analyzed using one-way anova and multiple differences test. Identification of the content

of the chloroform fraction eluated with methanol-ammonia, carried out by gas chroma-

tography mass spectrometry (GC-MS). The results showed that all three doses showed a

decrease in blood sugar levels significantly at day 7 , but the decline has not reach normal

levels. Blood sugar levels drop significantly and the decline has reached normal occurred on

day 14. Dominant content of the chloroform fraction eluated with methanol-ammonia is

a class of unsaturated fatty acid chain length is Hexanoic acid, C16H1202, hexadecanoic

acid methyl ester, C17H3402, 9-octadecenoic acid methyl ester, C19H3602, Oleic acid

butyl ester, C22H4202, 9-octadecenoic acid, C19H3602, Octadecanoic acid, C18H3602,

1,2-Benzendicarboxylic acid, C8H604, and Aplysterylacetate, C31H5202. Another result

is that Nordextromethorphan alkaloid compound, Cl 7H23NO. The conclusion is chloro-

form fraction of ciplukan herb has antidiabetic effects and has a class of compounds con-

tent of unsaturated fatty acids, Aplysterylacetate and alkaloid Nordextromethorphan.

Published at: Vol 9, No 1 (2012) pages: 20-30

DOI: 10.7454/psr.v9i1.3361

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