Vol 9, No 2 (2012) > Articles >

Uji Penghambatan Aktivitas alfa-glukosidase Ekstrak dan Fraksi Daun Antidesma montanum Blume

Nofiantini Nofiantini , Berna Elya , Azizahwati Azizahwati



alpha-Glucosidase inhibitor has known to be a therapeutic agent for diabetes mellitus (DM)

treatment, especially type 2 DM. Based on previous studies. There are various plants

that have the effect of inhibiting the activity of a-glucosidase, one of which is garu

leaves (Antidesma montanum Blume). This research aimed to get the fraction which

had the highest Il-glucosidase inhibiting activity from ethanol extract of garu leaves

and identify the chemical compounds from the most active fraction. Simplisia powder

was extracted by maseration using 80% ethanol then fractionated using n-hexane, ethyl

acetate, and methanol. Inhibitory activity test was performed by measuring absorbance

of p-nitrophenol, which produced by reaction between Il-glucosidase and p-nitrophenyl-

u-l)-glucopyranoside, using microplate reader at 405 nm. The result showed that ethyl

acetate fraction have the best Il-glucosidase inhibitory activity with IC50 values 138.38

ppm. The test of enzyme kinetics showed that ethyl acetate fraction inhibited competitively.

The phytochemical screening showed that ethyl acetate fraction of garu leaves contained

glycosides, tannins, and terpenes.

Keywords: a-glucosidase; diabetes mellitus; garu leaves

Published at: Vol 9, No 2 (2012) pages: 67-74

DOI: 10.7454/psr.v9i2.3353

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