Vol 2, No 3 (2015) > Articles >

Toksisitas Ekstrak Etanol Mangifera foetida L. Sebagai Pengkelat Besi Ditinjau dari LD50 dan Komponen Sel Darah

Tri Wahyuni , Santi Purna Sari , Ari Estuningtyas , HJ Freisleben



Ethanolic extract of bacang mango leaves (Mangifera foetida L.) was reported could decrease blood iron concentration of iron overloaded-Sprague dawley rats. The aim of this experiment was to determine acute toxicity (LD50) value of ethanolic extract of M. foetida by weil method and its effect to blood component. This study was conducted by employing a complete random design using 25 male and 25 female mice of DDY strain which was divided into 5 groups. The first until fourth group were administered by the aquadest suspension of ethanolic extract of M. foetida with dose variation, orally. The fifth group was control group that administered by aquadest only. The LD50 was determined by the total of death on all group. LD50 value of the extract showed no death in the biggest doses (13.013 g/kg). The examination was continued with measured blood count (erythrocytes, trombocytes, leukocytes, and haemoglobin concentration). It was shown that the extract at dose 1.626; 3.253; 6.506 and 13.013 g/kg unchanged the blood count measurement. It is concluded that Mangifera foetida L. leaf was not toxic, but could not change the blood count measurement.


Keywords: Mangifera foetida; iron overload; acute toxicity; LD50; blood count

Published at: Vol 2, No 3 (2015) pages: 124-134

DOI: 10.7454/psr.v2i3.3345

Access Counter: 2058 views

Full PDF Download


Anonim. (2000). Parameter Standar Umum Ekstrak Tumbuhan Obat. Cetakan Pertama. Jakarta: Departemen Kesehatan Republik Indonesia Direktorat Jenderal Pengawasan Obat dan Makanan Direktorat Pengawasan Obat Tradisional.

Brittenham GM. (2003). Iron chelators and iron toxicity. Alcohol. 2003;30:151-8.

Anonim. (1995). Farmakope Indonesia, Ed IV. Jakarta: Departemen Kesehatan Republik Indonesia.

Franck, C Lu. (1995). Toksikologi Dasar: Asas, Organ Sasaran, dan Penilaian Risiko Edisi 2 (Edi Nugroho, Penerjemah.). Jakarta: UI Press.

Gonza´lez JE, Rodrı´guez MD, Rodeiro I, Morffi J, Guerra E, Leal F, et al. (2007). Lack of in vivo embryotoxic and genotoxic activities of orally administered stem bark aqueous extract of Mangifera indica L. (Vimang®). Food and Chemical Toxicology, 45, 2526–2532.

Harmita & Maksum Radji. (2005). Buku Ajar Analisis Hayati Ed.2. Depok: Departemen Farmasi FMIPA UI.

Haśková P, Koubková L, Vávrová A, Macková E, Hruśková K, Kovaŕíková P, et al. (2011). Comparison of various iron chelators used in clinical practice as protecting agents against catecholamine-induced oxidative injury and cardiotoxicity. Toxicology, 289:122– 31.

Liu ZD, Hider RC. (2002). Design of iron chelators with therapeutic application. Coordination Chemistry Reviews, 232:151-71.

Nishiyama K, Choi YA, Honsho C, Eiadthong W, Yonemori K. (2006). Application of genomic in situ hybridization for phylogenetic study between Mangifera indica L. and eight wild species of Mangifera. Scientia Horticulturae, 110:114–17.

Pardo-Andreu G, Delgado R, Velho JA, Curti C, Vercesi AE. (2005). Iron complexing activity of mangiferin, a naturally occurring glucosylxanthone, inhibit mitochondrial lipid peroxidation induced by Fe2+-citrate. European Journal of Pharmacology, 513:47-55.

Pardo-Andreu GL, Cavalheiro RA, Dorta DJ, Naal Z, Delgado R, Vercesi AE, et al. (2007). Fe(III) shifts the mitochondria permeability transition-eliciting capacity of mangiferin to protection of organelle. The Journal of Pharmacology and Experimental Therapeutics, 320:646-53.

Purwaningsih EH, Hanani E, Amalia P, Krisnamurti DG. (2011). The chelating effect of Mangifera foetida water extract on serum thalassemic patient. J Indon Med Assoc, 61(8):321-5.

Rodeiro I, Donato MT, Jime´nez N, Garrido G, Delgado R, Go´mez-Lecho´n MJ. (2007). Effects of Mangifera indica L. aqueous extract (Vimang) on primary culture of rat hepatocytes. Food and Chemical Toxicology, 45,2506–12.

Siah CW, Trinder D, Olynnyk JK. (2005). Iron overload. Clinica Chimica Acta, 358:24-36.

Soebrata G. (2001). Penentuan Laboratorium Klinis. Jakarta:Dian Rakyat.

Szuber N, Buss JL, Soe-Lin S, Felfly H, Trudel M, Ponka P. (2008). Alternative treatment paradigm for thalassemia using iron chelators. Experimental Hematology, 36,773-85.

Wong C, Richardson DR. (2003). β-Thalassaemia: emergence of new and improved iron chelators for treatment. The International Journal of Biochemistry & Cell Biology, 35, 1144-9.