Vol 2, No 2 (2015) > Articles >

Uji Toksisitas Akut Dan Efek Antiinflamasi Ekstrak Metanol dan Ekstrak n-Heksana Daun Pepaya (Carica papaya L)

Tahara Dilla Santi

 

Abstract:

The acute toxicity test and antiinflammatory effect of methanolic and n-hexane extracts Carica papaya L leaf have been studied. The results of phytochemical screening show that papaya leaf contains alkaloid, steroid, and flavonoid compound. Flavonoid and alkaloid extracted from methanolic extract of papaya leaf and steroid extracted from n-hexane extract of papaya leaf. The acute toxicity test of methanolic and n-hexane extracts Carica papaya L leaf have been studied on rat with giving a single dose of samples. The aim of this work was to investigate the behavioral responses (pharmacological profile), the development of body weight and mortality for 14 days. The results of acute toxicity test after giving dose of 250 mg/kgbw, 500 mg/kgbw and 1000 mg/kgbw of methanolic and n-hexane extracts of leaves of Carica papaya L to males rat showed no animal died and significant toxic effect. In the study antiinflammatory effects in white rat with carragenan induced 1%, and to determine   an effective dose of extract papaya to decrease the edema volume of rat foot. The test was done using rat hind paw edema or esthablished an artificial inflammation in left foot of white male rats. The treatments were carried out on six groups, the positive control group was administered with Indomethacin 10 mg/kgbw per oral, the negative control group was administered with CMC 1%, and the extract groups were administered with 200 mg/kgbw and 400 mg/kgbw of methanolic and n-hexane extracts Carica papaya L leaf. Edema volume were measured every half hour for 5 hours using a pletismometer digital. Obtained data were analyzed by Kruskal-Wallis test followed Mann- Whitney test with confident level was 95%. The result of this study indicate that papaya leaf extract can reduce edema volume of rat foot.


Keywords: acute toxicity, antiinflammatory, Carica papaya L leaves, indomethacin.

Published at: Vol 2, No 2 (2015) pages: 101-114

DOI: 10.7454/psr.v2i2.3341


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References:

Hargono, D. Obat Analgetik dan Antiinflamasi Nabati. Cermin Dunia Kedokteran No. 129. 2000. Tersedia dari: http://www.kalbe.co.id/files/cdk/13ObatAnalgetikdan AntiinflamasiNabati129.

Warisno. Budidaya Pepaya. Yogyakarta: Kanisius ; 2003

Owoyele BV, Adebukola OM, Funmilayo AA, Soladoye AO. Anti-inflammatory activities of ethanolic extract of Carica papaya leaves. J. Inflammopharmacology, 2008;16:168-173.

Imaga NA, Gbenle GO, Okochi VI, Adenekan S, Duro-Emmanuel T, Oyeniyi B, et al. Phytochemical and antioxidant nutrient constituents of Carica papaya and Parquetina nigrescens extracts. Scientific Research and Essays 2010; 5(16): 2201-2205.

Dalimartha S. Care Your Self. Cetakan pertama. Jakarta: Penebar Plus; 2008.

Simon K, Kerry B. Principles and Pracice of Phytotheraphy. Modern Herbal Medicine. New York: Churchill Livingstone. 2000

Cuzzorcrea S. Increased Carragenan-Induced Acute Lung Inflamation In Old Rats, Immunology. 2005;115(2): 253-261.

Siswanto A, Nurulita NA. Daya Antiinflamasi Infus Daun Mahkota Dewa (Phaleria macrocarpa Scheff. Boerl) pada Tikus Putih (Rattus norvegicus) Jantan. (Prosiding Seminar Nasional TOI XXVII): 2005

Miller AL. Antioxidant flavonoids: structure, function, and clinical usage. Alternative Medicine Review, 1, 103; 1996

Katzung BG. Farmakologi dasar dan Klinik. Jakarta: Penerbit Salemba; 2002.

Patil BS, Miller EG. Inhibition of Oral Carcinogenesis by Citrus Flavonoids. Nutrition and Cancer 2008; 60(1): 69–74