Vol 2, No 1 (2015) > Articles >

Aktivitas Antioksidan Ekstrak, Fraksi dan Golongan Senyawa Kimia Daun Premna oblongata Miq.

Katrin Katrin , Atika Bendra

 

Abstract: Indonesia has many kinds of plants which can be used as antioxidant sources. This study is focused on antioxidant activity of Cincau Perdu leaves extract (Premna oblongata Miq.) by1,1-Diphenyl-2-picrylhydrazyl (DPPH) methods. Premna oblongata Miq.leaves was extracted using n-hexane, ethyl acetate and methanol. The IC50 value of methanol extract as the highest activity as antioxidantwas 20.01 μg/mL. The extract which had the highest antioxidant activity were fractionated by accelerated column chromatography and were earned 6 combination fractions. The antioxidant activity of each combination fractions were tested by DPPH methods. Fraction-5 showed the highest activity as antioxidant with IC50 23.51 μg/mL. The compounds of the active fractions were flavonoid, glikon, saponin and tanin.

Keywords: Antioxidant, DPPH, Premna oblongata Miq

Published at: Vol 2, No 1 (2015) pages: 21-31

DOI: 10.7454/psr.v2i1.3332


Access Counter: 185 views

Full PDF Download

References:

Andarwulan, N., Wijaya, H., dan Cahyono, D.T. (1996). Aktivitas Antioksidan dari Daun Sirih (Piper betle L).Teknologi dan Industri Pangan VII, 29-30.

Blois, M.S. (1958). Antioxidant Determinations By The Use Of A Stable FreeRadical Nature, 181, 1199- 1200.

Departemen Kesehatan Republik Indonesia (1995). Materia Medika Indonesia Jilid VI. Jakarta: Departemen Kesehatan Republik Indonesia.

Direktorat Pengawasan Obat Tradisional.(2000). Parameter Standar Umum Ekstrak Tumbuhan Obat Cetakan Pertama. Departemen KesehatanRepublik Indonesia.

Isnindar., Setyowat, E.P., dan Wahyuono, S. (2011). Aktivitas Antioksidan Daun Kesemek (Diospyros kaki L.F) Dengan Metode DPPH (2,2-Difenil- Pikrilhidrazin). Majalah Obat tradisional.

Molineux, P. (2004). The Use of The Stable Free Radical Diphenyl Picrylhydrazil(DPPH) for Estimating Antioxidant Activity. Songklankarin J. Sci.Technol., 26 (2), 211-219.

Nurdin., Kusharto, C.M., Tanziha, I., dan Januwati, M. (2009). Kandungan Kandungan Klorofil Berbagai Jenis Daun Tanaman dan Cu-Turunan Klorofil SertaKarakteristik Fisiko-Kimianya. Jurnal Gizi dan Pangan.

Pitojo, S., dan Sumiati. (2005). Cincau: Cara Pembuatan dan Variasi Olahan.Agromedia Pustaka, Jakarta.

Rajnarayana, K., Ajitha M., Gopireddy G., dan Giriprasad, V. (2011). Comperative Antioxidant Pharmacy& Technology.

Yu, L. (2008). Wheat Antioxidants. United States Of America: Wiley

Zakaria, F.R. (1996). Sintesis Senyawa Radikal dan Elektrofil Dalam Oleh Komponen Pangan Reaksi Biomolekuler, Dampak Terhadap Kesehatan dan Penangkalan. Prosiding Seminar. Bogor: Pusat Studi Pangan dan Gizi IPB.