Vol 3, No 1 (2016) > Articles >

Uji Penghambatan Xantin Oksidase Secara In Vitro Pada Ekstrak Kulit Rambutan

Nurul Eka Putri , Rissyelly Rissyelly , Marista Gilang Mauldina

 

Abstract:

Hyperuricemia is a condition have higher uric acid levels that can cause a cumulation uric acid crystals in the tissues. Xanthine oxidase is an enzyme which catalyze the oxidation of hypoxanthine into xanthine and into uric acid.Therefore, the inhibition of xanthine oxidase will reduce ammount of uric acid. This research aims to determine xanthine oxidase inhibition activity and also to identify chemical constituent group of extract Rambutan (Nephelium lappaceum Linn.) skin. Rambutan skin was extracted by graded maceration using a three solvent, based on polarity the solvent are n-hexane, ethyl acetate and methanol. The test of inhibition xanthine oxidase activity was using a spectrophotometer at λ = 274.79 nm, pH 7.8, substrate concentration of xanthine 0.15 mM and an incubation temperature of 30° C. Inhibition on Allopurinol as positive control has IC50 0.15 µg/mL. The result showed that methanol extract of Rambutan skin had the highest inhibition percentage with IC50 3.71 µg/mL. Phytochemical screening showed that the most active extract methanol from rambutan skin contain flavonoids, saponins, tannins and terpenoids.

 


Keywords: xanthine oxidase, hyperuricemia, inhibition activity, spectrophotometry, Nephelium lappaceum Linn.

Published at: Vol 3, No 1 (2016) pages: 12-20

DOI: 10.7454/psr.v3i1.3222


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References:

Azmi, S., Jamal, P., & Amid, A. (2012). Xantine Oxidase inhibitory activity from potental malaysian medicinal plan as remedie for gout. international food research journal, 19(1), 156-159.

Chung, S., Wen, L., Yi, J., Du, Y., Kuei, F., Wen, C., et al. (2012). Prevalence of hyperuricemia and its association with antihypertensive treatment in. International Journal of Cardiology, 156, 41-46.

Cos, P., Callome, M., Hu, J., Cimanga, K., Van Poel, B., Pieters, L., et al. (1998). Structure Activity Relationship and Classification of as Inhibitors of Xanthine Oxydase and Superoxide cavengers. journal Nat. Prod, 61-76.

Dalimartha , S. (2003). Atlas Tumbuhan Obat Indonesia jilid 3. Jakarta: Puspa suara.

Dalimartha, S. (2005). Atlas Tumbuhan Obat Indonesia Jilid 4. Jakarta: Puspa Suara.

Departemen Kesehatan Republik Indonesia. (1989). Materia Medika Indonesia jilid V. Jakarta: Departemen Kesehatan Republik Indonesia.

Departemen Kesehatan Republik Indonesia. (1995). Farmakope Indonesia edisi IV. Jakarta: Departemen Kesehatan Republik Indonesia.

Departemen Kesehatan Republik Indonesia. (1995). Materia Medika Indonesia jilid VI. Jakarta: Departemen Kesehatan Republik Indonesia.

Dipiro, J., Talbert, L., Yee, C., Matzke, R., Wells, G., & Posey, L. (2008). Pharmacotherapy,Seventh edition. New York: McGraw-Hill Companies, Inc.

Harborne, J. (1987). Metode Fitokimia Penuntun Cara Modern Mengekstraksi Tumbuhan. Bandung: ITB.

Kilburn, J., Thitilerdecha, N., Terawutgulgulrag, A., & Rakariyatham, N. (2010). Identification of Major Phenolic Compounds from Nephelium lappaceum L. and Their Antioxidant Activities. Journal Molecules, 15(3), 1453-1465.

Mitaal, A., Philips, S., Loveday, B., & Windsor, J. (2008). The potential role for xantin oxidase inhibition in major intra-abdominal surgery. World J. Surg, 32, 288-295..

Nasrul, E., & Sofitri. (2012). Hiperurisemia pada pra diabetes. Jurnal kesehatan Andalas, 1(2).

Owen, P., & Johns, T. (1999). Xanthine oxidase inhibitory activity of Northeastern North American Plant remedie used for gout. Journal of Ethnopharmacology, 64, 146-160.

Prihatman, K. (2000). Budidaya Pertanian: Rambutan. Dipetik Agustus 2015, 15, dari Sistem Informasi Manajemen Pembangunan di Perdesaan, Jakarta: http://www.warintek.ristek.go.id/pertanian/rambutan.pdf

Saleh, M., M, M., W, E., & Hatmoko, D. (2007). Determinasi dan morfologi buah eksotis potensial di lahan rawa. Dipetik Agustus 15, 2015, dari Balai Penelitian Pertanian Lahan Rawa Banjar Baru: http://balittra.litbang.pertanian.go.id/eksotik/Monograf%20-%207.pdf

Sudoyo, A., Satiyohadi, B., Alwi, I., K, M., & Setiati, S. (2006). Ilmu Penyakit Dalam (4 ed.). Jakarta: Departemen Ilmu Penyakit Dalam Fakultas Kedokteran Universitas Indonesia.

Thitilertdecha, N., Teerawutgulrag, A., & Rakariyatham, N. (2008). Antioxidant and Antibacterial Activities Nephelium lappaceum L. Extracts. Food Science and Technology, Elsevier, 41.

Umamaheswari, M., Asok , K., Somasundaran , A., Sivashanmugam, T., Subhadradevi, V., & Ravi, T. (2007). Xanthine oxidase inhibitory activity of some indian medial plant. Journal of Ethnopharmacology, 109, 547-551.

Wang, S., Liaob, J., Zhena, W., Chuc, F., & Chang, S. (2008). Eseential oil from leaves Cinnamomum osmophloem acts as a xanthine oxidase inhibitor and reduces the serum uric acid levels in oxonate induced mice. Journal of Phytomedicine, 15, 940-945.